Validation of self-collected buccal swab and saliva as a diagnostic tool for COVID-19.
Validation of self-collected buccal swab and saliva as a diagnostic tool for COVID-19.
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DOI：
10.1016/j.ijid.2020.12.080
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Chan JKY
中科院分区：
文献类型：
--
作者：
Ku CW;Shivani D;Kwan JQT;Loy SL;Erwin C;Ko KKK;Ng XW;Oon L;Thoon KC;Kalimuddin S;Chan JKY
有效管理严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）需要进行大规模检测，以识别和隔离感染携带者。自行使用咽拭子和唾液收集是目前卫生保健工作者（HCW）收集鼻咽拭子（NPS）的方便、无痛和安全的替代方法。对过去7天内通过NPS检测出SARS-CoV-2阳性的42名参与者进行了一项横断面单中心研究。进行实时聚合酶链反应（RT-PCR），并获得每个测试的周期阈值（Ct）。计算唾液样本和口腔拭子的阳性协议百分比（PPA）、阴性协议百分比（NPA）和总体协议百分比（OA），并与NPS进行比较。在42名参与者中，有73.8%（31/42）的人在三种检测中的任何一种检测中呈阳性。参考NPS，唾液检测PPA为66.7%，NPA为91.7%，OA为69.0%；口腔拭子PPA 56.7%, NPA 100%, OA 73.8%。自我收集的唾液测试和口腔拭子与hcw收集的NPS只有中等程度的一致。可通过唾液检测或口腔拭子对SARS-CoV-2进行初步筛查，如果检测呈阴性，则需要确认NPS，以避免假阴性，最大限度地减少不适，并降低向社区和卫生保健工作者传播的风险。
Effective management of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires large-scale testing to identify and isolate infectious carriers. Self-administered buccal swab and saliva collection are convenient, painless, and safe alternatives to the current healthcare worker (HCW)-collected nasopharyngeal swab (NPS). A cross-sectional single-centre study was conducted on 42 participants who had tested positive for SARS-CoV-2 via an NPS within the past 7 days. Real-time polymerase chain reaction (RT-PCR) was performed and cycle threshold (Ct) values were obtained for each test. The positive percent agreement (PPA), negative percent agreement (NPA), and overall agreement (OA) were calculated for the saliva samples and buccal swabs, and compared with NPS. Among the 42 participants, 73.8% (31/42) tested positive by any one of the three tests. With reference to NPS, the saliva test had PPA 66.7%, NPA 91.7%, and OA 69.0%; the buccal swab had PPA 56.7%, NPA 100%, and OA 73.8%. Self-collected saliva tests and buccal swabs showed only moderate agreement with HCW-collected NPS. Primary screening for SARS-CoV-2 may be performed with a saliva test or buccal swab, with a negative test warranting a confirmatory NPS to avoid false-negatives, minimize discomfort, and reduce the risk of spread to the community and HCWs.
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