Cell viability in intervertebral disc under various nutritional and dynamic loading conditions: 3d finite element analysis.
Cell viability in intervertebral disc under various nutritional and dynamic loading conditions: 3d finite element analysis.
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DOI：
10.1016/j.jbiomech.2012.08.044
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发表时间：
2012-11-15
影响因子：
2.4
通讯作者：
Gu WY
中科院分区：
文献类型：
--
作者：
Zhu Q;Jackson AR;Gu WY
在这项研究中，一个新的细胞密度模型，并纳入制定的机械-电化学混合物理论研究的影响，剥夺营养供应的边界源，变性，和动态加载椎间盘（IVD）的细胞活力，使用有限元方法。边界源的营养供应的剥夺是通过减少CEP和AF边界的营养水平来模拟的。在CEP和AF边界处具有100%、75%、60%、50%和30%正常营养水平的病例被建模。施加不同振幅（u=10%± 2.5%，±5%）和频率（f=1，10，20周期/天）组合的无限制轴向正弦动态压缩。退化的IVD是用改变的材料特性建模的。细胞密度随边界营养水平的降低而显著降低。细胞死亡主要在中平面上的NP-AF界面附近开始。在非退化的IVD中，动态负荷没有导致细胞密度的变化，因为葡萄糖水平没有下降到细胞存活的最低值以下;在退化的IVD中，我们发现增加频率和幅度都导致更高的细胞密度，因为动态压缩促进了营养物质的扩散，从而增加了IVD细胞周围的营养水平。新的计算模型可用于定量预测在各种营养和机械条件下细胞在IVD内何时何地开始死亡。
In this study, a new cell density model was developed and incorporated into the formulation of the mechano-electrochemical mixture theory to investigate the effects of deprivation of nutrition supply at boundary source, degeneration, and dynamic loading on the cell viability of intervertebral disc (IVD) using finite element methods. The deprivation of nutrition supply at boundary source was simulated by reduction in nutrition level at CEP and AF boundaries. Cases with 100%, 75%, 60%, 50% and 30% of normal nutrition level at both CEP and AF boundaries were modeled. Unconfined axial sinusoidal dynamic compressions with different combinations of amplitude (u=10%±2.5%, ±5%) and frequency (f=1, 10, 20 cycle/day) were applied. Degenerated IVD was modeled with altered material properties. Cell density decreased substantially with reduction of nutrition level at boundaries. Cell death was initiated primarily near the NP–AF interface on the mid-plane. Dynamic loading did not result in a change in the cell density in non-degenerated IVD, since glucose levels did not fall below the minimum value for cell survival; in degenerated IVDs, we found that increasing frequency and amplitude both resulted in higher cell density, because dynamic compression facilitates the diffusion of nutrients and thus increases the nutrition level around IVD cells. The novel computational model can be used to quantitatively predict both when and where cells start to die within the IVD under various kinds of nutritional and mechanical conditions.
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影响因子：
3.8
作者：
Gu, WY;Yao, H;Flagler, D
通讯作者：
Flagler, D
影响因子：
3
作者：
Bibby, SRS;Jones, DA;Urban, JPG
通讯作者：
Urban, JPG
影响因子：
3
作者：
BERNICK, S;CAILLIET, R
通讯作者：
CAILLIET, R
影响因子：
3.5
作者：
Ateshian, Gerard A.
通讯作者：
Ateshian, Gerard A.
影响因子：
2.8
作者：
Iatridis, JC;Kumar, S;Mow, VC
通讯作者：
Mow, VC