Effect of PACAP/PAC1R on Follicle Development of Djungarian Hamster (Phodopus sungorus) with the Variation of Ambient Temperatures.
Effect of PACAP/PAC1R on Follicle Development of Djungarian Hamster (Phodopus sungorus) with the Variation of Ambient Temperatures.
复制标题
作者：
环境温度影响了野生动物的生理活动，如繁殖。垂体腺苷环化酶激活多肽(PACAP)及其受体(PAC1R)通过影响环境温度变化下的卵泡发育来调节哺乳动物的生殖。然而，PACAP/PAC1R对太阳鱼生殖的影响尚不清楚。本研究探讨了在不同环境温度下，PACAP/PAC1R与卵泡发育相关的卵泡刺激素(FSH)之间的关系，从而最终影响太阳鱼的繁殖。低温(8℃、14℃)和高温(29℃)对生长卵泡和有腔卵泡的发育、PACAP/PAC1R表达和FSH血清浓度均有抑制作用。PACAP/PAC1R的PKA/PKG和PKC磷酸化位点可能通过cAMP-PKA及其下游信号通路参与FSH的合成。PACAP/PAC1R的表达水平与生长卵泡数、有腔卵泡数、血清FSH浓度、有腔卵泡数呈显著正相关。结论：(1)PACAP/PAC1R在进化上是保守的，并通过主要功能元件发挥作用；(2)温度依赖性的卵泡发育与PACAP/PAC1R的表达和血清FSH浓度有关。因此，PACAP/PAC1R和FSH参与了不同温度下卵泡的发育。这些结果不仅为太阳鱼繁殖的研究提供了工作依据，也为种群动态平衡的调控提供了理论依据。在太阳鱼中，垂体腺苷酸环化酶激活多肽(PACAP)及其受体(PAC1R)、卵泡刺激素(FSH)与卵泡发育的关系尚不清楚。在本研究中，我们发现在低温(8℃，14℃)和高温(29℃)下，生长卵泡和有腔卵泡的发育受到抑制。同时，与21℃相比，在8℃、14℃和29℃的环境温度下，PACAP/PAC1R的表达和卵泡刺激素(FSH)的血清浓度显著降低。因此，环境温度可能影响PACAP/PAC1R的表达和FSH的合成，从而参与卵泡的发育。此外，PACAP/PAC1R具有主要的功能元件，包括PKA/PKG和PKC磷酸化位点，可能通过cAMP-PKA及其下游信号通路参与FSH的合成。PACAP/PAC1R的表达水平与生长卵泡数、有腔卵泡数、血清FSH浓度、有腔卵泡数呈显著正相关。而PACAP/PAC1R的表达水平与血清FSH浓度无明显相关性，提示PACAP/PAC1R与FSH之间存在复杂的信号转导途径。综上所述，环境温度影响PACAP/PAC1R的表达和血清FSH浓度。PACAP/PAC1R的表达和血清FSH浓度与卵泡发育密切相关，提示它们参与卵泡发育，最终影响太阳鱼的繁殖。本研究为进一步研究太阳鱼生殖调控机制奠定了基础。
Ambient temperature has affected the physiological activities of wild animals, such as reproduction. Pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptor (PAC1R) regulate reproduction in mammals via influencing follicle development under ambient temperature variations. However, the effect of PACAP/PAC1R on the reproduction of Phodopus sungorus remains unclear. In this study, we explored the relationship between PACAP/PAC1R and follicle-stimulating hormone (FSH), involved in follicle development, at different ambient temperatures, which will ultimately influence the reproduction of Phodopus sungorus. The development of growing follicles and antral follicles were inhibited at low (8 °C, 14 °C) and high (29 °C) temperatures as well as PACAP/PAC1R expression and FSH serum concentration. The PKA/PKG and PKC phosphorylation sites of PACAP/PAC1R may be involved in the pathway of FSH synthesis through cAMP-PKA and its downstream signal pathway. Moreover, there was a significant positive correlation between the expression levels of PACAP/PAC1R and the number of the growing and antral follicles, as well as the serum FSH concentration and the number of antral follicles. In conclusion: (1) PACAP/PAC1R is evolutionarily conservative and exerts functions through major functional elements; (2) the temperature-dependent follicle development is correlated with the expression of PACAP/PAC1R and the serum FSH concentration. Therefore, PACAP/PAC1R and FSH are involved in the follicle development at different temperatures. These results not only provide a working basis for the study of Phodopus sungorus reproduction, but also provide a theoretical basis for the regulation of population dynamic equilibrium. In Phodopus sungorus, the relationship between pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptor (PAC1R), follicle-stimulating hormone (FSH), and follicle development remains unclear. In this study, we found that the development of growing follicles and antral follicles were inhibited at low (8 °C, 14 °C) and high (29 °C) temperatures. Meanwhile, PACAP/PAC1R expression and follicle-stimulating hormone (FSH) serum concentration significantly decreased during ambient temperatures of 8 °C, 14 °C and 29 °C compared to 21 °C. Thus, ambient temperature may influence the expression of PACAP/PAC1R and the synthesis of FSH for involvement in follicle development. Moreover, PACAP/PAC1R had major functional elements including PKA/PKG and PKC phosphorylation sites, which may involve in the pathway of FSH synthesis through cAMP-PKA and its downstream signal pathway. Moreover, there was a significant positive correlation between the expression levels of PACAP/PAC1R and the number of the growing and antral follicles, as well as the serum FSH concentration and the number of antral follicles. However, there was no significant correlation between the expression levels of PACAP/PAC1R and the serum FSH concentration, indicating a complicated pathway between PACAP/PAC1R and FSH. In conclusion, ambient temperature affects the expression of PACAP/PAC1R and the serum FSH concentration. The expression of PACAP/PAC1R and the serum FSH concentration are correlated with follicle development, which implies that they are involved in follicle development, which will ultimately influence the reproduction of Phodopus sungorus. This study can lay the foundation for future investigation on the regulation mechanism of reproduction in Phodopus sungorus.
登录
查看更多内容
影响因子：
--
作者：
FREHN, JL;LIU, CC
通讯作者：
LIU, CC
褪黑素对热应激下绵羊颗粒细胞增殖和凋亡的影响
影响因子：
5.6
作者：
Fu Y;He CJ;Ji PY;Zhuo ZY;Tian XZ;Wang F;Tan DX;Liu GS
通讯作者：
Liu GS
影响因子：
4.8
作者：
Abel, MH;Wootton, AN;Charlton, HM
通讯作者：
Charlton, HM
影响因子：
2.7
作者：
Egbert JR;Yee SP;Jaffe LA
通讯作者：
Jaffe LA
影响因子：
9.8
作者：
Caro SP;Schaper SV;Hut RA;Ball GF;Visser ME
通讯作者：
Visser ME