Effects of melatonin on the proliferation and apoptosis of sheep granulosa cells under thermal stress.
Effects of melatonin on the proliferation and apoptosis of sheep granulosa cells under thermal stress.
复制标题
褪黑素对热应激下绵羊颗粒细胞增殖和凋亡的影响
DOI：
10.3390/ijms151121090
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发表时间：
2014-11-14
影响因子：
5.6
通讯作者：
Liu GS
中科院分区：
文献类型：
--
作者：
Fu Y;He CJ;Ji PY;Zhuo ZY;Tian XZ;Wang F;Tan DX;Liu GS
卵母细胞和体细胞之间的相互作用在卵泡发育和卵母细胞成熟的调控中起着至关重要的作用。因此，颗粒细胞凋亡导致卵泡闭锁。在这项研究中，绵羊颗粒细胞培养的热应激诱导凋亡，褪黑激素（MT）的潜在影响，评估其对热诱导的颗粒细胞损伤。结果表明，颗粒细胞殖民地形成效率（CFE）明显降低热处理组（19.70% ± 1.29%）与对照组（26.96% ± 1.81%）比较，差异有统计学意义（P < 0.05）（热应激组56.16% ± 13.95%vs22.80% ± 12.16%，p < 0.05）。褪黑激素（10 - 7 M）显著降低了颗粒细胞中热应激引起的负面影响。这种减少通过这些细胞的CFE改善和凋亡率降低来指示。褪黑素对热应激颗粒细胞的保护作用不被其膜受体拮抗剂luzindole所抑制。机制探索表明，褪黑素（10 - 7 M）下调p53和上调Bcl-2和LHR基因表达的颗粒细胞在热应激下。本研究为褪黑素对热应激下颗粒细胞保护作用的分子机制提供了证据。
The cross-talk between oocyte and somatic cells plays a crucial role in the regulation of follicular development and oocyte maturation. As a result, granulosa cell apoptosis causes follicular atresia. In this study, sheep granulosa cells were cultured under thermal stress to induce apoptosis, and melatonin (MT) was examined to evaluate its potential effects on heat-induced granulosa cell injury. The results demonstrated that the Colony Forming Efficiency (CFE) of granulosa cells was significantly decreased (heat 19.70% ± 1.29% vs. control 26.96% ± 1.81%, p < 0.05) and the apoptosis rate was significantly increased (heat 56.16% ± 13.95%vs. control 22.80% ± 12.16%, p < 0.05) in granulosa cells with thermal stress compared with the control group. Melatonin (10−7 M) remarkably reduced the negative effects caused by thermal stress in the granulosa cells. This reduction was indicated by the improved CFE and decreased apoptotic rate of these cells. The beneficial effects of melatonin on thermal stressed granulosa cells were not inhibited by its membrane receptor antagonist luzindole. A mechanistic exploration indicated that melatonin (10−7 M) down-regulated p53 and up-regulated Bcl-2 and LHR gene expression of granulosa cells under thermal stress. This study provides evidence for the molecular mechanisms of the protective effects of melatonin on granulosa cells during thermal stress.
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DOI：
10.1186/1477-7827-10-49
发表时间：
2012-06-29
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Agarwal A;Aponte-Mellado A;Premkumar BJ;Shaman A;Gupta S
通讯作者：
Gupta S
影响因子：
4.8
作者：
Jiang, XJ;Wang, XD
通讯作者：
Wang, XD
影响因子：
1.7
作者：
Garcia-Ispierto, I.;Abdelfatah, A.;Lopez-Gatius, F.
通讯作者：
Lopez-Gatius, F.
L-苯丙氨酸对妊娠早期小鼠胚胎子宫着床的免疫和抗氧化作用
影响因子：
2
作者：
Dong, Yulan;Bai, Yongping;Yang, Hongliang
通讯作者：
Yang, Hongliang
影响因子：
1.7
作者：
Ishikawa, S.;Machida, R.;Tanemura, K.
通讯作者：
Tanemura, K.