Virus-induced gene silencing in Nicotiana benthamiana triggered by heterologous gene sequences from Viola philippica
Virus-induced gene silencing in Nicotiana benthamiana triggered by heterologous gene sequences from Viola philippica
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烟草中病毒诱导的基因沉默是由紫花地丁的异源基因序列触发的
DOI：
10.32615/bp.2019.018
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发表时间：
2019-01
影响因子：
1.5
通讯作者：
Chao-ying He
中科院分区：
文献类型：
--
作者：
Qiao-xia Li;Juan Wang;Sheng Zheng;Ning Yang;Kun Sun;Chao-ying He
病毒诱导的基因沉默（VIGS）是功能基因组学的一个特别有用的工具。在本研究中，我们试图利用这一技术来推断基因的功能，从紫花地丁烟草脆裂病毒。(TRV)构建体首先，来自V. philippica的八氢番茄红素去饱和酶基因（VpPDS）被沉默，并且观察到局部叶片漂白，但没有表现出系统效应，从而限制了TRV介导的基因沉默在受体植物中的利用。然而，当VpPDS序列用作触发物时，我们观察到本氏烟草中的系统性基因沉默，从而表明异源基因序列可以引起基因沉默。然后，我们研究了B类MADS-box基因家族中来自V. philippica的基因PISTILLATA（VpPI）的作用，该基因调控雄蕊和花瓣的身份和发育。以VpPI的编码区为触发因子，我们测定了相应GLOBOSA旁系同源物在N. benthamiana（NbGLO_1和NbGLO_2）的雄蕊到心皮的转化和花冠变形。benthamiana表明VpPI为NbGLO基因。functioned.as而VpPI的3 '非翻译区（3' UTR）和NbGLO基因的3 '非翻译区（3' UTR）下调了各自相应的基因，几乎不产生花的同源异型改变。本工作为基因沉默的基因特异性探针设计提供了一个更好的理解，同时也表明异源序列触发的VIGS是一种研究直向同源基因功能保守性的有效方法。
Virus-induced gene silencing (VIGS) is a particularly useful tool for functional genomics. In the present study, we.attempted to utilize this technology to infer the function of genes from Viola philippica using a tobacco rattle virus.(TRV) construct. Firstly, the phytoene desaturase gene from V. philippica (VpPDS) was silenced, and local leaf bleaching.was observed but did not exhibit systemic effects, thereby limiting utilization of TRV-mediated gene silencing in the.recipient plant. However, we observed systemic gene silencing in Nicotiana benthamiana when the VpPDS sequence.was used as a trigger, thereby suggesting that heterologous gene sequences could elicit gene silencing. We then.investigated the role of gene PISTILLATA from V. philippica (VpPI) of the B-class MADS-box gene family, which.regulates the identity and development of stamens and petals. Using the coding region of VpPI as triggers, we determined.the gene silencing efficiency of the corresponding GLOBOSA paralogs in N. benthamiana (NbGLO1 and NbGLO2), and.we observed stamen-to-carpel transformation and distorted corollas in N. benthamiana suggesting that VpPI functioned.as the NbGLO gene. However, the 3'-untranslated region (3'UTR) of VpPI and each 3'UTR of the NbGLO genes.downregulated its own corresponding gene, hardly producing floral homeotic alterations. This work provides a better.understanding of gene-specific probe design for gene silencing as well as shows that heterologous sequence-triggered.VIGS is an efficient way to investigate functional conservation of orthologous genes.
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影响因子：
--
作者：
S. P. Dinesh-Kumar;R. Anandalakshmi;R. Marathe;Michael Schiff;Yule Liu
通讯作者：
S. P. Dinesh-Kumar;R. Anandalakshmi;R. Marathe;Michael Schiff;Yule Liu
DOI：
10.1111/j.1365-313x.2006.02720.x
发表时间：
2006-05
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
J. Sundström;N. Nakayama;K. Glimelius;V. Irish
通讯作者：
J. Sundström;N. Nakayama;K. Glimelius;V. Irish
影响因子：
7.2
作者：
Ratcliff, F;Martin-Hernandez, AM;Baulcombe, DC
通讯作者：
Baulcombe, DC
影响因子：
1.2
作者：
Gao, Xiquan;Britt, Robert C.;He, Ping
通讯作者：
He, Ping
影响因子：
7.2
作者：
Brigneti, G;Martín-Hernández, AM;Jones, JDG
通讯作者：
Jones, JDG