Identification of genes involved in cell wall biogenesis in grasses by differential gene expression profiling of elongating and non-elongating maize internodes.
Identification of genes involved in cell wall biogenesis in grasses by differential gene expression profiling of elongating and non-elongating maize internodes.
复制标题
DOI：
10.1093/jxb/err045
复制
发表时间：
2011-06
影响因子：
6.9
通讯作者：
Donnison IS
中科院分区：
文献类型：
--
作者：
Bosch M;Mayer CD;Cookson A;Donnison IS
尽管禾草作为食物、饲料和能源作物具有重要的经济意义，但人们对控制其细胞壁合成、组装和重塑的基因知之甚少。这里提供了一个详细的转录组分析，允许鉴定参与草细胞壁生物发生的基因。使用玉米寡核苷酸阵列的差异基因表达谱，用于鉴定伸长节间的差异表达基因，其中包含具有初生细胞壁合成的细胞，以及刚刚停止伸长的节间，其中许多细胞正在沉积次生细胞壁物质。这是为数不多的专门针对禾草细胞壁相关基因鉴定的研究之一。分析发现了新的候选基因在草的初生和次生细胞壁生物发生中的作用。结果表明，在正常发育过程中参与细胞壁过程的许多蛋白质也在防御相关的细胞壁重塑事件中被招募。这项工作为候选基因参与细胞壁相关过程的功能测试提供了一个研究平台，增加了我们对草细胞壁生物发生及其调控的认识。由于目前正在开发几种草作为生物燃料生产的木质纤维素原料，因此对草细胞壁生物发生的理解的提高是及时的，因为它将促进有利于可持续粮食和生物燃料生产的性状的操纵。
Despite the economic importance of grasses as food, feed, and energy crops, little is known about the genes that control their cell wall synthesis, assembly, and remodelling. Here a detailed transcriptome analysis that allowed the identification of genes involved in grass cell wall biogenesis is provided. Differential gene expression profiling, using maize oligonucleotide arrays, was used to identify genes differentially expressed between an elongating internode, containing cells exhibiting primary cell wall synthesis, and an internode that had just ceased elongation and in which many cells were depositing secondary cell wall material. This is one of only a few studies specifically aimed at the identification of cell wall-related genes in grasses. Analysis identified new candidate genes for a role in primary and secondary cell wall biogenesis in grasses. The results suggest that many proteins involved in cell wall processes during normal development are also recruited during defence-related cell wall remodelling events. This work provides a platform for studies in which candidate genes will be functionally tested for involvement in cell wall-related processes, increasing our knowledge of cell wall biogenesis and its regulation in grasses. Since several grasses are currently being developed as lignocellulosic feedstocks for biofuel production, this improved understanding of grass cell wall biogenesis is timely, as it will facilitate the manipulation of traits favourable for sustainable food and biofuel production.
登录
查看更多内容
影响因子：
27.5
作者：
Cao, Pei-Jian;Bartley, Laura E.;Ronald, Pamela C.
通讯作者：
Ronald, Pamela C.
影响因子：
6.9
作者：
Bomal, Claude;Bedon, Frank;Caron, Sebastien;Mansfield, Shawn D.;Levasseur, Caroline;Cooke, Janice E. K.;Blais, Sylvie;Tremblay, Laurence;Morency, Marie-Josee;Pavy, Nathalie;Grima-Pettenati, Jacqueline;Seguin, Armand;MacKay, John
通讯作者：
MacKay, John
影响因子：
5.2
作者：
Caparros-Ruiz, David;Fornale, Silvia;Rigau, Joan
通讯作者：
Rigau, Joan
影响因子：
11.6
作者：
Diet, Anouck;Link, Bruce;Ringli, Christoph
通讯作者：
Ringli, Christoph
影响因子：
7.2
作者：
Dauwe, Rebecca;Morreel, Kris;Boerjan, Wout
通讯作者：
Boerjan, Wout