Involvement of Pinus taeda MYB1 and MYB8 in phenylpropanoid metabolism and secondary cell wall biogenesis: a comparative in planta analysis.
Involvement of Pinus taeda MYB1 and MYB8 in phenylpropanoid metabolism and secondary cell wall biogenesis: a comparative in planta analysis.
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DOI：
10.1093/jxb/ern234
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发表时间：
2008
影响因子：
6.9
通讯作者：
MacKay, John
中科院分区：
文献类型：
--
作者：
Bomal, Claude;Bedon, Frank;Caron, Sebastien;Mansfield, Shawn D.;Levasseur, Caroline;Cooke, Janice E. K.;Blais, Sylvie;Tremblay, Laurence;Morency, Marie-Josee;Pavy, Nathalie;Grima-Pettenati, Jacqueline;Seguin, Armand;MacKay, John
火炬松中两个R2 R3-MYB基因的参与，PtMYB 1和PtMYB 8在植物体内苯丙素代谢和次生细胞壁发生中的作用。这些松树MYB组成型过表达（OE）的云杉glauca（Moench）Voss，用作异源针叶树表达系统。形态学，组织学，化学（木质素和可溶性酚），和转录分析，即微阵列和逆转录定量PCR（RT-qPCR）用于广泛的表型MYB过表达云杉幼苗。体细胞胚萌发后，两种转基因植物的根生长都减少。增强的木质素沉积也是一个共同的特征，但异位次生细胞壁沉积与PtMYB 8-OE更强烈地相关。微阵列和RT-qPCR数据显示，每种MYB的过表达导致编码参与木质素单体合成的苯丙素酶的许多基因的重叠上调，而几种细胞壁相关基因和其它MYB转录因子的失调与PtMYB 8-OE特异性相关。总之，结果表明MYB 1和MYB 8可能是针叶树次生细胞壁沉积中涉及的保守转录网络的一部分。
The involvement of two R2R3-MYB genes from Pinus taeda L., PtMYB1 and PtMYB8, in phenylpropanoid metabolism and secondary cell wall biogenesis was investigated in planta. These pine MYBs were constitutively overexpressed (OE) in Picea glauca (Moench) Voss, used as a heterologous conifer expression system. Morphological, histological, chemical (lignin and soluble phenols), and transcriptional analyses, i.e. microarray and reverse transcription quantitative PCR (RT-qPCR) were used for extensive phenotyping of MYB-overexpressing spruce plantlets. Upon germination of somatic embryos, root growth was reduced in both transgenics. Enhanced lignin deposition was also a common feature but ectopic secondary cell wall deposition was more strongly associated with PtMYB8-OE. Microarray and RT-qPCR data showed that overexpression of each MYB led to an overlapping up-regulation of many genes encoding phenylpropanoid enzymes involved in lignin monomer synthesis, while misregulation of several cell wall-related genes and other MYB transcription factors was specifically associated with PtMYB8-OE. Together, the results suggest that MYB1 and MYB8 may be part of a conserved transcriptional network involved in secondary cell wall deposition in conifers.
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针叶树R2R3-MYB转录因子：白云杉（Picea Glauca）的木制组织中的序列分析和基因表达。
影响因子：
5.3
作者：
Bedon, Frank;Grima-Pettenati, Jacqueline;Mackay, John
通讯作者：
Mackay, John
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
7.2
作者：
Gómez-Maldonado, J;Avila, C;Campbell, MM
通讯作者：
Campbell, MM
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
7.2
作者：
Goicoechea, M;Lacombe, E;Grima-Pettenati, J
通讯作者：
Grima-Pettenati, J