Isolation of circulating epithelial and tumor progenitor cells with an invasive phenotype from breast cancer patients.
Isolation of circulating epithelial and tumor progenitor cells with an invasive phenotype from breast cancer patients.
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DOI：
10.1002/ijc.24814
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发表时间：
2010-02-01
影响因子：
6.4
通讯作者：
Chen, Wen-Tien
中科院分区：
文献类型：
--
作者：
Lu, Janice;Fan, Tina;Zhao, Qiang;Zeng, Wei;Zaslavsky, Eva;Chen, John J.;Frohman, Michael A.;Golightly, Marc G.;Madajewicz, Stefan;Chen, Wen-Tien
最近的研究进展表明，肿瘤细胞内渗（进入循环）和转移发生在乳腺癌进展的非常早期。临床研究还说明了检测循环肿瘤细胞（CTC）在转移性乳腺癌患者结局中的潜在重要性。这些细胞是否表现出侵袭性并表达肿瘤干细胞或祖细胞标志物，转移表型的标志，是不太好的特点。为了检测具有侵袭性表型的CTC并探索其分子特征，我们应用了一种功能性细胞分离方法，称为胶原粘附基质（CAM）测定，作为富集和鉴定步骤。CAM涂层装置成功回收了掺入1 mL血液中的肿瘤细胞，回收率为54%±9%（n=18），纯度为0.5-35%，并在转移性乳腺癌患者的10/10份血液样本（100%产率）中检测到浸润性肿瘤细胞，范围为18至256个CTC/mL，平均值为126±25（平均值±SD）个CTC/mL。在28/54（52%）I-III期乳腺癌患者的血液样品中检测到CTC，平均计数为61个CTC/mL。此外，这些细胞的相对频率与早期乳腺癌患者的分期、淋巴结状态和生存相关。CAM捕获的细胞能够在培养物中繁殖。CAM捕获的细胞的基因表达和多重流式细胞术分析表明存在不同的CTC群体，包括上皮谱系和干细胞或祖细胞。因此，CAM启动的CTC检测为检查侵袭性和肿瘤祖细胞表型提供了优势。
Recent research advances show that tumor cell intravasation (entry into the circulation) and metastasis occur very early in breast cancer progression. Clinical studies also illustrate the potential importance of detection of circulating tumor cells (CTCs) in outcomes of patients with metastatic breast cancer. Whether these cells exhibit the invasiveness and express tumor stem or progenitor markers, hallmark of the metastatic phenotype, is less well characterized. To detect CTCs with the invasive phenotype and to explore their molecular features, we applied a functional cell separation method, called collagen adhesion matrix (CAM) assay, as enrichment and identification steps. The CAM-coated device successfully recovered tumor cells spiked in one mL of blood with a 54%±9% (n=18) recovery rate and 0.5-35% purity, and detected invasive tumor cells in 10/10 blood samples (100% yield) from patients with metastatic breast cancer with a range of 18 to 256 CTCs/mL and average of 126±25 (mean±SD) CTCs/mL. CTCs were detected in blood samples of 28/54 (52%) stage I-III breast cancer patients with a mean count of 61 CTCs/mL. Furthermore, the relative frequency of these cells correlated to the staging, lymph node-status and survival of patients with early stage breast cancer. CAM-captured cells were capable of propagation in culture. Gene expression and multiplex flow cytometric analyses on CAM-captured cells demonstrated the existence of distinct populations of CTCs including these of epithelial lineage and stem or progenitor cells. Thus, CAM-initiated CTC detection provides advantages for examining invasiveness and tumor progenitor phenotypes.
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影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
DOI：
10.1073/pnas.0404036101
发表时间：
2004-07-20
影响因子：
11.1
作者：
Krivacic, RT;Ladanyi, A;Bruce, RH
通讯作者：
Bruce, RH
影响因子：
11.5
作者：
Pierga, JY;Bonneton, C;Magdelénat, H
通讯作者：
Magdelénat, H
影响因子：
11.5
作者：
Ignatiadis, Michail;Kallergi, Galatea;Mavroudis, Dimitris
通讯作者：
Mavroudis, Dimitris
DOI：
10.1073/pnas.042372199
发表时间：
2002-02-19
影响因子：
11.1
作者：
Solakoglu, O;Maierhofer, C;Pantel, K
通讯作者：
Pantel, K