Bromopyrrole Alkaloids with the Inhibitory Effects against the Biofilm Formation of Gram Negative Bacteria.
Bromopyrrole Alkaloids with the Inhibitory Effects against the Biofilm Formation of Gram Negative Bacteria.
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溴吡咯生物碱对革兰氏阴性菌生物膜形成的抑制作用
DOI：
10.3390/md16010009
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发表时间：
2018-01-02
期刊：
影响因子：
5.4
通讯作者：
Lin W
中科院分区：
文献类型：
--
作者：
Sun J;Wu J;An B;Voogd NJ;Cheng W;Lin W
抗生物被膜法筛选出的柱花草马萨的正丁醇可溶性组分对细菌E.杆菌对活性组分进行色谱分离，得到32种溴吡咯生物碱，其中包括6种新生物碱，命名为stylisines A-F（1-6）。通过二维核磁共振（COSY、HMQC和HMBC）和高分辨电喷雾质谱（HRESIMS）数据的综合分析，确定了新生物碱的结构，并通过X射线衍射和电子圆二色谱（ECD）数据确定了其绝对构型。生物测定结果表明，phakellin生物碱，包括二溴异法克林和二溴phakellin，显着减少细菌的生物膜形成。杆菌本工作为抑制大肠杆菌生物被膜形成提供了一组新的天然支架。杆菌
Anti-biofilm assay guided fractionation of the marine sponge Stylissa massa revealed the butanol soluble fraction that was possessing the inhibitory activity toward the biofilm formation of bacterium E. coli. Chromatographic separation of the bioactive fraction resulted in the isolation of 32 bromopyrrole alkaloids, including six new alkaloids, named stylisines A–F (1–6). The structures of new alkaloids were established by comprehensive analyses of the two-dimensional (2D) NMR (COSY, HMQC, and HMBC) and the high resolution electrospray ionization mass spectroscopy (HRESIMS) data, while the absolute configurations were determined by the X-ray diffraction and the electronic circular dichroism (ECD) data. Bioassay results indicated that phakellin-based alkaloids, including dibromoisophakellin and dibromophakellin, significantly reduced the biofilm formation of the bacterium E. coli. Present work provided a group of new natural scaffolds for the inhibitory effects against the biofilm formation of E. coli.
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影响因子：
5.4
作者：
Forte B;Malgesini B;Piutti C;Quartieri F;Scolaro A;Papeo G
通讯作者：
Papeo G
影响因子：
1.8
作者：
Andrade, P;Willoughby, R;Kerr, RG
通讯作者：
Kerr, RG
影响因子：
5.1
作者：
Assmann, M;van Soest, RWM;Köck, M
通讯作者：
Köck, M
影响因子：
3.6
作者：
Ballard, T. Eric;Richards, Justin J.;Melander, Christian
通讯作者：
Melander, Christian
影响因子：
2.1
作者：
Fouad, Mostafa A.;Debbab, Abdessamad;Proksch, Peter
通讯作者：
Proksch, Peter