Argon plasma treatment of polystyrene microtiter wells. Chemical and physical characterisation by contact angle, ToF-SIMS, XPS and STM
Argon plasma treatment of polystyrene microtiter wells. Chemical and physical characterisation by contact angle, ToF-SIMS, XPS and STM
复制标题
聚苯乙烯微量滴定孔的氩等离子体处理。
DOI：
10.1016/s0927-7757(00)00497-0
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
S. Hibbert
中科院分区：
文献类型：
--
作者：
J. Davies;C. Nunnerley;Anneli C. Brisley;R. F. Sunderland;J. C. Edwards;P. Krüger;R. Knes;A. Paul;S. Hibbert
通过接触角测量、X射线光电子能谱 (XPS) 和飞行时间二次离子质谱 (ToF-SIMS) 评估了等离子体处理在聚苯乙烯微量滴定孔改性中的应用。接触角数据表明，等离子体处理的作用首先是清洁表面的挥发性污染物，增加疏水性，然后将氧官能团引入表面，降低疏水性。清洁效果似乎出现在治疗的最初几秒钟内，而氧化效果随着等离子体暴露的增加而增强。 XPS 和 ToF-SIMS 测量结果显示，表面氧浓度随着等离子体处理时间的延长而增加，同时芳香度也随之降低。扫描隧道显微镜（STM）成像表明，等离子体处理显着影响牛血清白蛋白（BSA）的吸附。未处理的表面表现出未发生 BSA 吸附的区域。这些区域的直径大小在 20 至 60 nm 之间。然而，等离子处理的表面没有表现出这样的区域，BSA 吸附在整个表面上似乎更加均匀。未处理表面上未发生 BSA 吸附的区域被认为本质上是碳氢化合物（挥发性），可能来自成型过程，在等离子体处理的最初几秒钟内被去除。
The use of plasma treatment for the modification of polystyrene microtiter wells has been evaluated by contact angle measurements, X-ray photoelectron spectroscopy (XPS), and time-of-flight secondary ion mass spectrometry (ToF-SIMS). The contact angle data suggests that the effect of plasma treatment is first to clean the surface of volatile contamination, increasing the hydrophobicity, and then to introduce oxygen functionality into the surface, decreasing the hydrophobicity. The cleaning effect appears to occur in the first few seconds of treatment while the oxygenation effect increases with increased exposure to the plasma. The XPS and ToF-SIMS measurements show increasing surface oxygen concentration with plasma treatment time, with a concomitant reduction in aromaticity. Scanning tunnelling microscopy (STM) imaging reveals that plasma treatment significantly affects the adsorption of bovine serum albumin (BSA). Untreated surfaces exhibited areas where no BSA adsorption occurred. These regions ranged in size between 20 and 60 nm in diameter. Plasma treated surfaces, however, exhibited no such areas, with BSA adsorption appearing to be more uniform across the surface. The regions on the untreated surfaces where no BSA adsorption occurred are thought to be hydrocarbon (volatile) in nature possibly from the moulding process, which is removed in the first few seconds of plasma treatment.
将高亲和力肝素寡糖固定到射频等离子体改性聚乙烯上。
DOI：
10.1002/jbm.820270614
发表时间：
1993
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
Yuan,S;Szakalas-Gratzl,G;Ziats,NP;Jacobsen,DW;Kottke-Marchant,K;Marchant,RE
通讯作者：
Marchant,RE