Molecular Detection and Typing of Pathogenic Leptospira in Febrile Patients and Phylogenetic Comparison with Leptospira Detected among Animals in Tanzania.
Molecular Detection and Typing of Pathogenic Leptospira in Febrile Patients and Phylogenetic Comparison with Leptospira Detected among Animals in Tanzania.
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DOI：
10.4269/ajtmh.19-0703
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发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Crump JA
中科院分区：
文献类型：
--
作者：
Allan KJ;Maze MJ;Galloway RL;Rubach MP;Biggs HM;Halliday JEB;Cleaveland S;Saganda W;Lwezaula BF;Kazwala RR;Mmbaga BT;Maro VP;Crump JA
需要分子数据来提高我们对非洲钩端螺旋体病流行病学的理解，并确定人类感染源。我们应用分子生物学方法鉴定坦桑尼亚发热住院患者中感染的钩端螺旋体种类和基因型，并将其与坦桑尼亚动物中检测到的钩端螺旋体基因型进行比较，以推断人类感染的潜在来源。我们进行了lipL32实时PCR检测急性期血浆，血清和尿液样本中的致病性钩端螺旋体的存在下，从研究参与者血清学确诊的钩端螺旋体病和参与者谁已经死亡的发热性疾病。还进行了钩端螺旋体血培养。在阳性标本中，我们进行了种特异性PCR，并将参与者钩端螺旋体secY序列与钩端螺旋体参考序列和先前从坦桑尼亚动物中获得的序列进行了比较。我们在111份参与者血液样本中的4份（3.6%）中检测到钩端螺旋体DNA。我们检测到博氏钩端螺旋体（1名参与者，25.0%）、问号钩端螺旋体（1名参与者，25.0%）和克氏钩端螺旋体（1名参与者，25.0%）（1名[25%]未确定）。对L. borgpetersenii和L.从参与者中检测到的kirschneri基因型与在当地牲畜物种中检测到的基因型密切相关但不同。我们的研究结果表明，各种各样的钩端螺旋体物种是造成人类感染。尽管我们的分析表明人和牲畜中发现的钩端螺旋体基因型之间存在密切关系，但需要继续努力从人类钩端螺旋体病病例中获得更多的钩端螺旋体遗传物质，以帮助优先考虑控制的钩端螺旋体物种和基因型。
Molecular data are required to improve our understanding of the epidemiology of leptospirosis in Africa and to identify sources of human infection. We applied molecular methods to identify the infecting Leptospira species and genotypes among patients hospitalized with fever in Tanzania and compared these with Leptospira genotypes detected among animals in Tanzania to infer potential sources of human infection. We performed lipL32 real-time PCR to detect the presence of pathogenic Leptospira in acute-phase plasma, serum, and urine samples obtained from study participants with serologically confirmed leptospirosis and participants who had died with febrile illness. Leptospira blood culture was also performed. In positive specimens, we performed species-specific PCR and compared participant Leptospira secY sequences with Leptospira reference sequences and sequences previously obtained from animals in Tanzania. We detected Leptospira DNA in four (3.6%) of 111 participant blood samples. We detected Leptospira borgpetersenii (one participant, 25.0%), Leptospira interrogans (one participant, 25.0%), and Leptospira kirschneri (one participant, 25.0%) (one [25%] undetermined). Phylogenetic comparison of secY sequence from the L. borgpetersenii and L. kirschneri genotypes detected from participants was closely related to but distinct from genotypes detected among local livestock species. Our results indicate that a diverse range of Leptospira species is causing human infection. Although our analysis suggests a close relationship between Leptospira genotypes found in people and livestock, continued efforts are needed to obtain more Leptospira genetic material from human leptospirosis cases to help prioritize Leptospira species and genotypes for control.
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