Classic maximum entropy recovery of the average joint distribution of apparent FRET efficiency and fluorescence photons for single-molecule burst measurements.

Classic maximum entropy recovery of the average joint distribution of apparent FRET efficiency and fluorescence photons for single-molecule burst measurements.
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用于单分子爆发测量的表观 FRET 效率和荧光光子的平均联合分布的经典最大熵恢复。

DOI:
10.1021/jp209861u
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发表时间:
2012
期刊:
The journal of physical chemistry. B
影响因子:
--
通讯作者:
Johnson,CareyK
Johnson,CareyK
中科院分区:
--
文献类型:
--
作者:
DeVore,MatthewS;Gull,StephenF;Johnson,CareyK

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我们描述了一种使用经典的最大熵分析单分子Fo Prster共振能量转移(FRET)猝发测量的方法。经典的最大熵决定了贝叶斯推断的联合概率描述的总荧光光子和表观FRET效率。用模拟数据和荧光染料标记的DNA对该方法进行了验证。最可能的联合分布可以被边缘化,以获得荧光光子的总体分布和表观FRET效率分布。这种方法被证明是理想的用于确定FRET标记的生物分子的距离分布,它成功地预测了恢复的分布的形状。
We describe a method for analysis of single-molecule Förster resonance energy transfer (FRET) burst measurements using classic maximum entropy. Classic maximum entropy determines the Bayesian inference for the joint probability describing the total fluorescence photons and the apparent FRET efficiency. The method was tested with simulated data and then with DNA labeled with fluorescent dyes. The most probable joint distribution can be marginalized to obtain both the overall distribution of fluorescence photons and the apparent FRET efficiency distribution. This method proves to be ideal for determining the distance distribution of FRET-labeled biomolecules, and it successfully predicts the shape of the recovered distributions.
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