A cytosolic phospholipase A2 from potato tissues appears to be patatin.
A cytosolic phospholipase A2 from potato tissues appears to be patatin.
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来自马铃薯组织的胞质磷脂酶 A2 似乎是 patatin。
DOI：
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发表时间：
1996
影响因子：
4.9
通讯作者：
K. Kawakita
中科院分区：
文献类型：
--
作者：
K. Senda;H. Yoshioka;N. Doke;K. Kawakita
磷脂酶（PL） A2参与了马铃薯抗性反应的信号转导，这种抗性反应是通过接种晚疫病菌疫霉（Phytophthora infestans）的不亲和小种，或用真菌激发剂菌丝壁成分处理而诱导的（Kawakita et al. 1993）。本研究对马铃薯块茎可溶性部分中的PLA2进行了纯化。以下结果表明，该酶实际上是patatin:(1)纯化酶的分子质量为40 kDa，与patatin相同；(2)纯化酶的pI约为4.75，与patatin的pI相当；(3)纯化酶的氨基末端氨基酸序列与patatin具有高度的同源性。马铃薯蛋白被认为是马铃薯块茎中的一种储存蛋白，它已被证明具有酯酶活性。然而，patatin的其他酶活性和功能尚不清楚。我们研究了纯化的patatin的PLA活性。该蛋白的PLA2活性远高于PLA1活性，尽管该蛋白具有PLA1活性和PLA2活性。以sn-2位亚油酸的磷脂酰胆碱为底物时，酶的PLA2活性特别明显。磷脂酰胆碱与棕榈酸、油酸和花生四烯酸在sn-2位的活性较低。
Phospholipase (PL) A2 is involved in signal transduction in the resistance reaction that is induced in potato by inoculation of an incompatible race of Phytophthora infestans, the late blight fungus, or by treatment with fungal elicitor hyphal wall components (Kawakita et al. 1993). In this study, PLA2 in the soluble fraction from potato tuber was purified. The following results suggested that the enzyme was, in fact, patatin: (1) the molecular mass of the purified enzyme was 40 kDa, the same as that of patatin; (2) the pI of the purified enzyme was approximately 4.75, which corresponds to that of patatin; and (3) the amino-terminal amino acid sequence of the purified enzyme showed a high degree of homology to that of patatin. Patatin is known as a storage protein of the potato tuber and it has been shown to have esterase activity. However, other enzymatic activities and the function(s) of patatin are unknown. We investigated the PLA activities of the purified patatin. The PLA2 activity of the patatin was much higher than the PLA1 activity, even though the protein exhibited both activities. The PLA2 activity of the enzyme was particularly apparent when phosphatidylcholine with linoleic acid at the sn-2 position was used as substrate. Lower activity was observed with phosphatidylcholine with palmitic acid, oleic acid and arachidonic acid at the sn-2 position.
人中性粒细胞合成血小板激活因子和白三烯 B4 似乎需要蛋白激酶 C 活性。
DOI：
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发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
McIntyre,TM;Reinhold,SL;Prescott,SM;Zimmerman,GA
通讯作者：
Zimmerman,GA
磷脂酶 A2 和脂蛋白脂肪酶水解荧光磷脂底物。
DOI：
10.1016/0006-291x(84)91479-7
发表时间：
1984
影响因子：
3.1
作者：
Wittenauer,LA;Shirai,K;Jackson,RL;Johnson,JD
通讯作者：
Johnson,JD
马铃薯主要块茎蛋白 patatin 的两类基因在块茎和根中的表达存在差异。
影响因子：
14.9
作者：
Pikaard,CS;Brusca,JS;Hannapel,DJ;Park,WD
通讯作者：
Park,WD