Automated fluid delivery from multiwell plates to microfluidic devices for high-throughput experiments and microscopy.
Automated fluid delivery from multiwell plates to microfluidic devices for high-throughput experiments and microscopy.
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DOI：
10.1038/s41598-018-24504-x
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发表时间：
2018-04-18
影响因子：
4.6
通讯作者：
Albrecht DR
中科院分区：
文献类型：
--
作者：
Lagoy RC;Albrecht DR
高通量生物和化学实验通常使用多孔板或微流体装置以紧凑的形式分析大量独立样品。多孔板可方便地在静态流体环境中筛选化学库，而微流体装置在流量控制和动力学方面提供了巨大的灵活性。以简单且自动化的方式连接这些平台将引入新的高通量实验功能，例如具有精确曝光时间的复合屏幕。尽管目前将微流体装置与多孔板集成的方法仍然昂贵或技术复杂，但我们在此提出了一种简单的开源机器人系统，该系统可以通过单个连接的入口顺序输送液体。我们首先通过自动将 96 种染料溶液输送到微流体装置来表征可靠性和性能。接下来，我们在一次实验中测量了数十只活体线虫中两种感觉神经元类型的体内神经活动的气味剂量反应曲线。然后，我们鉴定了抑制光遗传学诱发的神经活动的化学物质，展示了整个生物体神经调节的功能筛选平台。最后，我们自动化了 85 分钟、十步的细胞染色方案。总之，这些例子表明，我们的系统可以通过经济地桥接高通量系统的两个常见元素：多孔板和微流体，来自动化各种方案并加速实验。
High-throughput biological and chemical experiments typically use either multiwell plates or microfluidic devices to analyze numerous independent samples in a compact format. Multiwell plates are convenient for screening chemical libraries in static fluid environments, whereas microfluidic devices offer immense flexibility in flow control and dynamics. Interfacing these platforms in a simple and automated way would introduce new high-throughput experimental capabilities, such as compound screens with precise exposure timing. Whereas current approaches to integrate microfluidic devices with multiwell plates remain expensive or technically complicated, we present here a simple open-source robotic system that delivers liquids sequentially through a single connected inlet. We first characterized reliability and performance by automatically delivering 96 dye solutions to a microfluidic device. Next, we measured odor dose-response curves of in vivo neural activity from two sensory neuron types in dozens of living C. elegans in a single experiment. We then identified chemicals that suppressed optogenetically-evoked neural activity, demonstrating a functional screening platform for neural modulation in whole organisms. Lastly, we automated an 85-minute, ten-step cell staining protocol. Together, these examples show that our system can automate various protocols and accelerate experiments by economically bridging two common elements of high-throughput systems: multiwell plates and microfluidics.
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评估14个有机溶剂和载体，用于筛查斑马鱼胚胎和幼虫。
影响因子：
3.7
作者：
Maes J;Verlooy L;Buenafe OE;de Witte PA;Esguerra CV;Crawford AD
通讯作者：
Crawford AD
影响因子：
8.8
作者：
Larsch J;Flavell SW;Liu Q;Gordus A;Albrecht DR;Bargmann CI
通讯作者：
Bargmann CI
DOI：
10.1007/978-1-4939-2842-2_12
发表时间：
2015-01-01
期刊：
C. ELEGANS: METHODS AND APPLICATIONS, 2ND EDITION
影响因子：
--
作者：
Lagoy, Ross C.;Albrecht, Dirk R.
通讯作者：
Albrecht, Dirk R.
一种新型的96固有形式的微间隙板，可在原发性肿瘤样品上进行药物反应分析。
影响因子：
4.6
作者：
Ma WY;Hsiung LC;Wang CH;Chiang CL;Lin CH;Huang CS;Wo AM
通讯作者：
Wo AM
影响因子：
16.6
作者：
Mondal, Sudip;Hegarty, Evan;Martin, Chris;Gokce, Sertan Kutal;Ghorashian, Navid;Ben-Yakar, Adela
通讯作者：
Ben-Yakar, Adela