Depletion of the SR-Related Protein TbRRM1 Leads to Cell Cycle Arrest and Apoptosis-Like Death in Trypanosoma brucei
Depletion of the SR-Related Protein TbRRM1 Leads to Cell Cycle Arrest and Apoptosis-Like Death in Trypanosoma brucei
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SR 相关蛋白 TbRRM1 的消耗导致布氏锥虫细胞周期停滞和细胞凋亡样死亡
作者：
G. Levy;Carolina P. Bañuelos;Analía G Níttolo;Gastón E Ortiz;Nicolás Mendiondo;G. Moretti;V. Tekiel;D. Sánchez
含精氨酸-丝氨酸（RS）结构域的蛋白是在RNA代谢中具有多种功能的RNA结合蛋白。在哺乳动物细胞中，这组蛋白质还涉及细胞周期和细胞凋亡的调节和协调。在锥虫（真核细胞谱系中的早期分支群）中，这组蛋白由3个成员代表，其中两个是SR蛋白，并且最近已显示参与rRNA加工以及前mRNA剪接和稳定性。在这里，我们报告了我们对第三个成员，SR相关蛋白TbRRM1的研究结果。在本研究中，我们发现TbRRM1的RNA干扰在T。布鲁氏原周期细胞导致细胞周期阻滞、与喷嘴表型相容的异常细胞伸长和通过类细胞分裂机制的细胞死亡。我们的研究结果扩大了锥虫RS结构域包含蛋白质在关键细胞过程中的作用，如细胞周期和类骨化症死亡，哺乳动物SR蛋白也发挥作用，从而表明在这个遗传上保守的蛋白质家族中具有保守的功能。
Arginine-Serine (RS) domain-containing proteins are RNA binding proteins with multiple functions in RNA metabolism. In mammalian cells this group of proteins is also implicated in regulation and coordination of cell cycle and apoptosis. In trypanosomes, an early branching group within the eukaryotic lineage, this group of proteins is represented by 3 members, two of them are SR proteins and have been recently shown to be involved in rRNA processing as well as in pre-mRNA splicing and stability. Here we report our findings on the 3rd member, the SR-related protein TbRRM1. In the present study, we showed that TbRRM1 ablation by RNA-interference in T. brucei procyclic cells leads to cell-cycle block, abnormal cell elongation compatible with the nozzle phenotype and cell death by an apoptosis-like mechanism. Our results expand the role of the trypanosomal RS-domain containing proteins in key cellular processes such as cell cycle and apoptosis-like death, roles also carried out by the mammalian SR proteins, and thus suggesting a conserved function in this phylogenetically conserved protein family.
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剪接进化的早期祖先：富含丝氨酸-精氨酸的克氏锥虫蛋白 (TcSR) 在顺式剪接中发挥功能。
影响因子：
1.5
作者：
Portal,Daniel;Espinosa,JoaquínM;Lobo,GuillermoS;Kadener,Sebastián;Pereira,ClaudioA;DeLaMata,Manuel;Tang,Zhaohua;Lin,Ren-Jang;Kornblihtt,AlbertoR;Baralle,FranciscoE;Flawiá,MirthaM;Torres,HéctorN
通讯作者：
Torres,HéctorN
影响因子：
3.3
作者：
Tu, XM;Wang, CC
通讯作者：
Wang, CC
有针对性地破坏一种重要的 RNA 结合蛋白会扰乱布氏锥虫的细胞分裂。
影响因子：
1.5
作者：
Manger,ID;Boothroyd,JC
通讯作者：
Boothroyd,JC
影响因子：
1.5
作者：
Siegel, T. Nicolai;Hekstra, Doeke R.;Cross, George A. M.
通讯作者：
Cross, George A. M.
影响因子：
16
作者：
Zhong, Xiang-Yang;Wang, Pingping;Han, Joonhee;Rosenfeld, Michael G.;Fu, Xiang-Dong
通讯作者：
Fu, Xiang-Dong