Golgi-to-ER retrograde transport prevents premature differentiation of Drosophila type II neuroblasts via Notch-signal-sending daughter cells.
Golgi-to-ER retrograde transport prevents premature differentiation of Drosophila type II neuroblasts via Notch-signal-sending daughter cells.
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DOI：
10.1016/j.isci.2023.108545
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发表时间：
2024-01-19
期刊：
影响因子：
5.8
通讯作者：
Wang, Su
中科院分区：
文献类型：
--
作者：
Zhang, Huanhuan;Rui, Menglong;Ma, Zhixin;Gong, Sifan;Zhang, Shuliu;Zhou, Qingxia;Gan, Congfeng;Gong, Wenting;Wang, Su
干细胞是异质性的，以产生器官发生所需的多种分化细胞类型;然而，不同地维持这些异质性干细胞的潜在机制尚未得到很好的理解。在这项研究中，我们确定高尔基体到内质网（ER）的逆行运输通过Notch信号特异性地维持II型成神经细胞（NB）。我们揭示了中间神经祖细胞（INPs），即II型NB的直接子细胞，提供Delta并充当NB生态位。INPs使用的Delta主要由NB产生，并且不对称地分配给INPs。阻断逆行转运导致INP数量减少，这降低了Notch活性并导致II型NB的过早分化。此外，Delta的减少可以抑制II型NB引起的肿瘤形成。我们的研究结果突出了高尔基体到ER逆行转运，Notch信号传导，干细胞生态位和融合之间的串扰，作为维持II型NB谱系自我更新的重要步骤。阻断RT通过降低Notch活性特异性影响II型NB INPs为NB提供Delta作为生态位II型NB产生的Delta不对称分布到INPs中生物科学;微生物学;细胞生物学
Stem cells are heterogeneous to generate diverse differentiated cell types required for organogenesis; however, the underlying mechanisms that differently maintain these heterogeneous stem cells are not well understood. In this study, we identify that Golgi-to-endoplasmic reticulum (ER) retrograde transport specifically maintains type II neuroblasts (NBs) through the Notch signaling. We reveal that intermediate neural progenitors (INPs), immediate daughter cells of type II NBs, provide Delta and function as the NB niche. The Delta used by INPs is mainly produced by NBs and asymmetrically distributed to INPs. Blocking retrograde transport leads to a decrease in INP number, which reduces Notch activity and results in the premature differentiation of type II NBs. Furthermore, the reduction of Delta could suppress tumor formation caused by type II NBs. Our results highlight the crosstalk between Golgi-to-ER retrograde transport, Notch signaling, stem cell niche, and fusion as an essential step in maintaining the self-renewal of type II NB lineage. Blocking RT specifically affects type II NBs by reducing Notch activity INPs provide Delta to NBs as niche The Delta generated by type II NBs was asymmetrically distributed into INPs Biological sciences; Microbiology; Cell biology
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