The promoter of the oocyte-specific gene, Oog1, functions in both male and female meiotic germ cells in transgenic mice.
The promoter of the oocyte-specific gene, Oog1, functions in both male and female meiotic germ cells in transgenic mice.
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DOI：
10.1371/journal.pone.0068686
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Minami N
中科院分区：
文献类型：
--
作者：
Ishida M;Okazaki E;Tsukamoto S;Kimura K;Aizawa A;Kito S;Imai H;Minami N
Oog1 是一种卵母细胞特异性基因，其表达在胚胎第 15.5 天 (E) 的小鼠卵母细胞中开启，同时大多数雌性生殖细胞停止增殖并进入减数分裂前期。在这里，我们表征了 Oog1 启动子，并表明由 Oog1 转录起始位点上游 2.7 kb 和 3.9 kb 区域驱动的转基因 GFP 报告基因表达重现了内在的 Oog1 表达模式。此外，3.9 kb 上游区域比 2.7 kb 区域表现出更强的转录活性，这表明在 3.9 kb 启动子内发现的额外 1.2 kb 区域中的调控功能可能是保守的。有趣的是，较长的启动子（3.9 kb）在雄性生殖细胞中也显示出很强的活性，从粗线期晚期的精母细胞到细长的精子细胞。这可能是由于近端启动子区域两个 CpG 位点的异常去甲基化所致。一种在 GFP 表达受到抑制的组织中高度甲基化，另一种仅在 Oog1pro3.9 雄性和雌性生殖细胞中完全去甲基化。这些结果表明，近端启动子区域的异常去甲基化在 3.9 kb Oog1 启动子的控制下诱导雄性生殖细胞异位表达。这是第一份表明性别依赖性基因表达根据启动子区域的长度和甲基化状态而改变的报告。此外，我们的结果表明，各个 CpG 位点甲基化程度不同，在调节启动子活性和基因转录中发挥不同的作用。
Oog1 is an oocyte-specific gene whose expression is turned on in mouse oocytes at embryonic day (E) 15.5, concomitant with the time when most of the female germ cells stop proliferating and enter meiotic prophase. Here, we characterize the Oog1 promoter, and show that transgenic GFP reporter expression driven by the 2.7 kb and 3.9 kb regions upstream of the Oog1 transcription start site recapitulates the intrinsic Oog1 expression pattern. In addition, the 3.9 kb upstream region exhibits stronger transcriptional activity than does the 2.7 kb region, suggesting that regulatory functions might be conserved in the additional 1.2 kb region found within the 3.9 kb promoter. Interestingly, the longer promoter (3.9 kb) also showed strong activity in male germ cells, from late pachytene spermatocytes to elongated spermatids. This is likely due to the aberrant demethylation of two CpG sites in the proximal promoter region. One was highly methylated in the tissues in which GFP expression was suppressed, and another was completely demethylated only in Oog1pro3.9 male and female germ cells. These results suggest that aberrant demethylation of the proximal promoter region induced ectopic expression in male germ cells under the control of 3.9 kb Oog1 promoter. This is the first report indicating that sex-dependent gene expression is altered according to the length and the methylation status of the promoter region. Additionally, our results show that individual CpG sites are differentially methylated and play different roles in regulating promoter activity and gene transcription.
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影响因子：
3.7
作者：
Merbs SL;Khan MA;Hackler L Jr;Oliver VF;Wan J;Qian J;Zack DJ
通讯作者：
Zack DJ
卵巢基因表达在没有图形的情况下，卵母细胞特异性转录因子。
影响因子：
--
作者：
Joshi, Saurabh;Davies, Holly;Sims, Lauren Porter;Levy, Shawn E.;Dean, Jurrien
通讯作者：
Dean, Jurrien
DOI：
10.1038/nrg2847
发表时间：
2010-08
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
10.5
作者：
Gazdag, Emese;Santenard, Angele;Torres-Padilla, Maria-Elena
通讯作者：
Torres-Padilla, Maria-Elena
DOI：
10.1530/acta.0.0620098
发表时间：
1969-01-01
期刊：
ACTA ENDOCRINOLOGICA
影响因子：
--
作者：
PETERS, H
通讯作者：
PETERS, H