Identification of N-linked carbohydrates from severe acute respiratory syndrome (SARS) spike glycoprotein.
Identification of N-linked carbohydrates from severe acute respiratory syndrome (SARS) spike glycoprotein.
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从严重的急性呼吸综合征（SARS）尖峰糖蛋白中鉴定N-连接的碳水化合物。
DOI：
10.1016/j.virol.2009.12.020
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发表时间：
2010-04-10
期刊：
影响因子：
3.7
通讯作者：
Rudd PM
中科院分区：
文献类型：
--
作者：
Ritchie G;Harvey DJ;Feldmann F;Stroeher U;Feldmann H;Royle L;Dwek RA;Rudd PM
从Vero E6细胞产生的SARS冠状病毒(SARS-CoV)刺突糖蛋白中释放N-糖链，采用基质辅助激光解吸电离(MALDI)质谱仪、负离子电喷雾碰撞诱导解离飞行时间质谱仪和外切糖苷酶消化正相高效液相色谱相结合的方法对其结构进行鉴定。主要的糖类是高甘露糖(Man5-9GlcNAc2)，杂化和双、三、四触角的复合体，有或没有二等分GlcNAc和核心岩藻糖。半乳糖残基少于全部含量的复杂多聚糖存在，唾液酸化可以忽略不计。用糖苷酶抑制剂N-丁基脱氧诺吉霉素(NB-DNJ)处理抑制N-糖链的加工，这从组成Glc3Man7-9GlcNAc2的糖链的出现中得到了证明。然而，一些复杂的多糖仍然存在，表明存在α-内切甘露糖苷酶。我们在组织培养中的数据表明，抑制N-聚糖的加工可能被认为是对抗SARS冠状病毒感染的一种治疗策略。
N-glycans were released from the SARS coronavirus (SARS-CoV) spike glycoprotein produced in Vero E6 cells and their structures were determined by a combination of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, negative ion electrospray collision-induced dissociation time-of-flight mass spectrometry and normal-phase high-performance liquid chromatography with exoglycosidase digestion. Major glycans were high-mannose (Man5–9GlcNAc2), hybrid and bi-, tri- and tetra-antennary complex with and without bisecting GlcNAc and core fucose. Complex glycans with fewer than the full complement of galactose residues were present and sialylation was negligible. Treatment with the glucosidase inhibitor N-butyl-deoxynojirimycin (NB-DNJ) inhibited N-glycan processing as evidenced by the appearance of glycans of composition Glc3Man7–9GlcNAc2. However, some complex glycans remained suggesting the presence of an α-endomannosidase. Our data in tissue culture indicate that inhibition of N-glycan processing may be considered as a therapeutic strategy against SARS CoV infections.
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普罗蛋白转化酶在严重急性呼吸综合征冠状病毒的加工和传播中的影响。
DOI：
10.1016/j.bbrc.2004.11.063
发表时间：
2005-01-21
影响因子：
3.1
作者：
Bergeron E;Vincent MJ;Wickham L;Hamelin J;Basak A;Nichol ST;Chrétien M;Seidah NG
通讯作者：
Seidah NG
影响因子：
64.8
作者：
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通讯作者：
Farzan M
DOI：
10.1016/j.jasms.2005.01.004
发表时间：
2005-05-01
影响因子：
3.2
作者：
Harvey, DJ
通讯作者：
Harvey, DJ
DOI：
10.1016/j.jasms.2005.01.005
发表时间：
2005-05-01
影响因子：
3.2
作者：
Harvey, DJ
通讯作者：
Harvey, DJ
DOI：
10.1074/mcp.m300048-mcp200
发表时间：
2003-05
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Krokhin O;Li Y;Andonov A;Feldmann H;Flick R;Jones S;Stroeher U;Bastien N;Dasuri KV;Cheng K;Simonsen JN;Perreault H;Wilkins J;Ens W;Plummer F;Standing KG
通讯作者：
Standing KG