Mutagenesis of a herpes simplex virus origin of DNA replication and its effect on viral interference.
Mutagenesis of a herpes simplex virus origin of DNA replication and its effect on viral interference.
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单纯疱疹病毒 DNA 复制起源的诱变及其对病毒干扰的影响。
DOI：
10.1099/0022-1317-66-1-31
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
N. Stow
中科院分区：
文献类型：
--
作者：
N. Stow
含有DNA片段克隆拷贝的超螺旋质粒分子被核酸酶S1优先在病毒插入物内的两个位置切割，所述DNA片段包括功能性单纯疱疹病毒1型DNA复制起点。构建了在这些位点插入分子接头的质粒，并对两个代表性质粒进行了分析，证明了在优选切割位置存在回文DNA序列。这些回文序列之一发生在一个90 bp的区域，其中的顺式作用序列的病毒起源功能所必需的先前已被定位。在这个位置插入一个接头废除了起源活动，证明了在DNA合成的起始回文序列内的一个重要作用。在转染试验中，含有DNA复制的功能性病毒起点的质粒显著干扰非缺陷病毒DNA的感染性，即使在没有病毒复制信号的情况下。灭活的起源大大降低了这种影响的DNA感染性，表明病毒干扰可能是介导的一种机制，涉及复制机制。
Supercoiled plasmid molecules containing cloned copies of a DNA fragment which includes a functional herpes simplex virus type 1 origin of DNA replication were cleaved preferentially at two positions within the viral insert by nuclease S1. Plasmids with molecular linker insertions at these sites were constructed, and analysis of two representative plasmids demonstrated the presence of palindromic DNA sequences at the preferred cleavage positions. One of these palindromic sequences occurred within a 90 bp region in which the cis-acting sequences essential for viral origin function had previously been located. Insertion of a linker at this position abolished origin activity, demonstrating an essential role for sequences within the palindrome in the initiation of DNA synthesis. In transfection assays, plasmids containing a functional viral origin of DNA replication markedly interfered with the infectivity of non-defective viral DNA even in the absence of viral encapsidation signals. Inactivation of the origin greatly reduced this effect on DNA infectivity, suggesting that viral interference may be mediated by a mechanism involving the replicative machinery.
SV40 的起源缺陷突变体。
DOI：
10.1101/sqb.1980.044.01.033
发表时间：
1980
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Gluzman,Y;Frisque,RJ;Sambrook,J
通讯作者：
Sambrook,J
细胞相关病毒胸苷激酶基因的疱疹病毒依赖性扩增和倒位，其两侧为病毒 a 序列，并与病毒 DNA 复制起点相连。
DOI：
10.1073/pnas.79.18.5626
发表时间：
1982
影响因子：
11.1
作者：
Mocarski,ES;Roizman,B
通讯作者：
Roizman,B
肿瘤抗原结合和DNA复制缺陷的猿猴病毒40起源的构建和分析。
DOI：
10.1073/pnas.77.11.6491
发表时间：
1980
影响因子：
11.1
作者：
Myers,RM;Tjian,R
通讯作者：
Tjian,R