Induced pluripotent stem cells have similar immunogenic and more potent immunomodulatory properties compared with bone marrow-derived stromal cells in vitro.
Induced pluripotent stem cells have similar immunogenic and more potent immunomodulatory properties compared with bone marrow-derived stromal cells in vitro.
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DOI：
10.2217/rme.14.29
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发表时间：
2014
影响因子：
2.7
通讯作者：
Fortier LA
中科院分区：
文献类型：
--
作者：
Schnabel LV;Abratte CM;Schimenti JC;Felippe MJ;Cassano JM;Southard TL;Cross JA;Fortier LA
比较诱导多能干细胞(IPSCs)和骨髓间充质干细胞(MSCs)在体外的免疫原性和免疫调节特性。从C3HeB/FEJ和C57BL/6J小鼠中分离出小鼠胚胎成纤维细胞(MEF)，并对其进行重编程以生成IPSCs。使用MHC相合和错配的应答白细胞和刺激因子白细胞、IPSCs或MSCs进行混合白细胞反应。为评估免疫原性，以IPSCs和MSCs作为反应性白细胞的刺激细胞。为了评估免疫调节特性，在刺激和应答白细胞存在的情况下培养IPSCs和MSCs。MEF作为对照。IPSCs具有与MSCs相似的免疫原性，但具有更强的免疫调节作用。MHC配型不合的白细胞与MHC配型的IPSCs共培养，与单独培养MHC配型不合的白细胞相比，反应性T细胞的增殖显著降低，并且与MSCs相比，反应性白细胞浓度更高。此外，MHC不相合的IPSCs与MHC不相合的白细胞共培养时，显著降低了应答者T细胞的增殖，而MHC不相合的MSCs没有。这些结果为考虑在再生医学和移植医学中使用IPSCs取代MSCs提供了重要信息。
To evaluate the in vitro immunogenic and immunomodulatory properties of induced pluripotent stem cells (iPSCs) compared with bone marrow-derived mesenchymal stromal cells (MSCs). Mouse embryonic fibroblasts (MEFs) were isolated from C3HeB/FeJ and C57BL/6J mice, and reprogrammed to generate iPSCs. Mixed leukocyte reactions were performed using MHC-matched and -mismatched responder leukocytes and stimulator leukocytes, iPSCs or MSCs. To assess immunogenic potential, iPSCs and MSCs were used as stimulator cells for responder leukocytes. To assess immunomodulatory properties, iPSCs and MSCs were cultured in the presence of stimulator and responder leukocytes. MEFs were used as a control. iPSCs had similar immunogenic properties but more potent immunomodulatory effects than MSCs. Co-culture of MHC-mismatched leukocytes with MHC-matched iPSCs resulted in significantly less responder T-cell proliferation than observed for MHC-mismatched leukocytes alone and at more responder leukocyte concentrations than with MSCs. In addition, MHC-mismatched iPSCs significantly reduced responder T-cell proliferation when co-cultured with MHC-mismatched leukocytes, while MHC-mismatched MSCs did not. These results provide important information when considering the use of iPSCs in place of MSCs in both regenerative and transplantation medicine.
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影响因子：
2.6
作者：
Bartholomew, A;Sturgeon, C;Hoffman, R
通讯作者：
Hoffman, R
影响因子：
--
作者：
Jones, EA;Kinsey, SE;McGonagle, D
通讯作者：
McGonagle, D
影响因子：
9.1
作者：
Chang, Ying-Jun;Zhao, Xiang-Yu;Huang, Xiao-Jun
通讯作者：
Huang, Xiao-Jun
影响因子：
4
作者：
Baddoo, M;Hill, K;Phinney, DG
通讯作者：
Phinney, DG
影响因子：
20.3
作者：
Aggarwal, S;Pittenger, MF
通讯作者：
Pittenger, MF