Quantitative and Physiological Analyses of Chloride Dependence of Growth of Halobacillus halophilus
Quantitative and Physiological Analyses of Chloride Dependence of Growth of Halobacillus halophilus
复制标题
嗜盐杆菌生长的氯依赖性的定量和生理分析
DOI：
10.1128/aem.64.10.3813-3817.1998
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发表时间：
1998
影响因子：
4.4
通讯作者：
V. Müller
中科院分区：
文献类型：
--
作者：
M. Roeßler;V. Müller
ABSTRACT A quantitative analysis of the Cl− dependence of growth of Halobacillus halophilus was performed. Optimal growth rates were obtained at Cl− concentrations of between 0.5 and 2.0 M, and the final yield was also strictly dependent on the Cl− concentration. Br− but not I−, SO42−, NO2−, SO2−, OCN−, SCN−, BO2−, or BrO3− could substitute for Cl−. To analyze the function of chloride, chloride concentration was determined. At low external Cl− (Cle−) concentrations, the growth rate was low and Cl− was excluded from the cytoplasm; increasing the Cle−concentration led to an increase in the growth rate and an energy-dependent uptake of Cl−, thus decreasing the Cle−/internal Cli− gradient from ≥10 at 0.1 M Cle− to a nearly constant value of 2 at Cle− concentrations which allowed optimal growth. Two membrane proteins with apparent molecular masses of 31 and 16 kDa which were identified to be specific for Cl−-grown cultures are possible candidates for a chloride uptake system.
利用脂质体、单克隆抗体和补体对霉菌毒素 T-2 进行均相免疫测定。
影响因子：
2.9
作者：
Ligler,FS;Bredehorst,R;Talebian,A;Shriver,LC;Hammer,CF;Sheridan,JP;Vogel,CW;Gaber,BP
通讯作者：
Gaber,BP