Absolute Quantitative MALDI Imaging Mass Spectrometry: A Case of Rifampicin in Liver Tissues.
Absolute Quantitative MALDI Imaging Mass Spectrometry: A Case of Rifampicin in Liver Tissues.
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绝对定量的MALDI成像质谱法：肝组织中利福平的一个病例。
DOI：
10.1021/acs.analchem.5b04409
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发表时间：
2016-02-16
影响因子：
7.4
通讯作者：
Caprioli, Richard M.
中科院分区：
文献类型：
--
作者：
Chumbley, Chad W.;Reyzer, Michelle L.;Allen, Jamie L.;Marriner, Gwendolyn A.;Via, Laura E.;Barry, Clifton E., III;Caprioli, Richard M.
基质辅助激光解吸/电离（MALDI）成像质谱（IMS）阐明薄组织切片中的分子分布。绝对像素到像素的定量仍然是一个挑战，主要是缺乏适当的分析方法的验证。在本工作中，同位素标记的内标物应用于组织切片，以最大限度地提高定量再现性，并产生准确的定量结果。我们已经开发了利福平（RIF），用于治疗结核病的抗生素的组织模型，并测试了不同的方法应用同位素标记的内标MALDI IMS分析。将标准品和随后的基质应用于组织切片，导致定量结果与使用组织提取物的HPLC-MS/MS获得的结果无统计学显著差异。对来自体内给药的动物的肝组织进行定量IMS实验。定量图像中的每个微点测量薄组织切片中RIF的局部浓度。从血管和汇管区周围检测到较低浓度。从这些测量中获得的定量值与从相同组织提取物中获得的HPLC-MS/MS结果相当（>90%相似性）。
Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) elucidates molecular distributions in thin tissue sections. Absolute pixel-to-pixel quantitation has remained a challenge, primarily lacking validation of the appropriate analytical methods. In the present work, isotopically labeled internal standards are applied to tissue sections to maximize quantitative reproducibility and yield accurate quantitative results. We have developed a tissue model for rifampicin (RIF), an antibiotic used to treat tuberculosis, and have tested different methods of applying an isotopically labeled internal standard for MALDI IMS analysis. The application of the standard and subsequently the matrix onto tissue sections resulted in quantitation that was not statistically significantly different from results obtained using HPLC-MS/MS of tissue extracts. Quantitative IMS experiments were performed on liver tissue from an animal dosed in vivo. Each microspot in the quantitative images measures the local concentration of RIF in the thin tissue section. Lower concentrations were detected from the blood vessels and around the portal tracts. The quantitative values obtained from these measurements were comparable (>90% similarity) to HPLC-MS/MS results obtained from extracts of the same tissue.
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影响因子：
4.3
作者：
Buck, Achim;Halbritter, Susanne;Walch, Axel
通讯作者：
Walch, Axel
影响因子：
7.4
作者：
Hankin, Joseph A.;Murphy, Robert C.
通讯作者：
Murphy, Robert C.
影响因子：
7.4
作者：
Fehniger, Thomas E.;Vegvari, Akos;Marko-Varga, Gyorgy
通讯作者：
Marko-Varga, Gyorgy
影响因子：
3.7
作者：
Nilsson A;Fehniger TE;Gustavsson L;Andersson M;Kenne K;Marko-Varga G;Andrén PE
通讯作者：
Andrén PE
影响因子：
2
作者：
Koeniger, Stormy L.;Talaty, Nari;Spanton, Stephen
通讯作者：
Spanton, Stephen