Pathogenicity study in sheep using reverse-genetics-based reassortant bluetongue viruses.
Pathogenicity study in sheep using reverse-genetics-based reassortant bluetongue viruses.
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DOI：
10.1016/j.vetmic.2014.09.012
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发表时间：
2014-11-07
影响因子：
3.3
通讯作者：
Roy, Polly
中科院分区：
文献类型：
--
作者：
Celma, Cristina C.;Bhattacharya, Bishnupriya;Eschbaumer, Michael;Wernike, Kerstin;Beer, Martin;Roy, Polly
使用反向遗传学来产生用于在动物中进行测试的抗性BTV病毒。两种结构蛋白和一种非结构蛋白参与致病性。蓝舌病的分子基础似乎非常复杂。蓝舌病是由呼肠孤病毒科的无包膜蓝舌病毒引起的一种重要的反刍动物疾病。目前，世界上已知26种不同的BTV血清型，其中BTV-8已被发现在家畜中表现出BT疾病的最致命表现之一。近年来，BTV-8在欧洲的入侵不仅导致绵羊而且也导致牛的显著发病率和死亡率。BTV-8发病机制的分子和遗传基础尚不清楚。为了了解BTV-8致病性的遗传基础，我们通过将最近分离的BTV-8的3个最可变的基因S2、S6和S10以不同组合替换到BTV-1的减毒株的骨架中来产生重组病毒。然后，在来自不同器官（肾脏和胸腺）的两种不同绵羊细胞系中分析这些抗性病毒的生长特征。在这两种细胞系中观察到每种抗性病毒的不同模式。为了确定这些病毒的致病性，BTV易感绵羊组感染这些病毒。这些数据表明，这两种不同血清型BTV-1和BTV-8的临床表现略有不同，并且当BTV-1包含BTV-8的所有3个基因组片段时，其表现与BTV-1不同。我们的研究结果还表明，BT疾病的分子基础是高度复杂的。
Use of reverse genetics to generate reassortant BTV viruses for testing in animals. Two structural and one non-structural proteins are involved in pathogenicity. Molecular basis of bluetongue disease appears to be highly complex. Bluetongue (BT) disease, caused by the non-enveloped bluetongue virus (BTV) belonging to the Reoviridae family, is an economically important disease that affects a wide range of wild and domestic ruminants. Currently, 26 different serotypes of BTV are recognized in the world, of which BTV-8 has been found to exhibit one of the most virulent manifestations of BT disease in livestock. In recent years incursions of BTV-8 in Europe have resulted in significant morbidity and mortality not only in sheep but also in cattle. The molecular and genetic basis of BTV-8 pathogenesis is not known. To understand the genetic basis of BTV-8 pathogenicity, we generated reassortant viruses by replacing the 3 most variable genes, S2, S6 and S10 of a recent isolate of BTV-8, in different combinations into the backbone of an attenuated strain of BTV-1. The growth profiles of these reassortant viruses were then analyzed in two different ovine cell lines derived from different organs, kidney and thymus. Distinct patterns for each reassortant virus in these two cell lines were observed. To determine the pathogenicity of these reassortant viruses, groups of BTV-susceptible sheep were infected with each of these viruses. The data suggested that the clinical manifestations of these two different serotypes, BTV-1 and BTV-8, were slightly distinct and BTV-1, when comprising all 3 genome segments of BTV-8, behaved differently to BTV-1. Our results also suggested that the molecular basis of BT disease is highly complex.
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影响因子：
3.7
作者：
Pérez de Diego AC;Athmaram TN;Stewart M;Rodríguez-Sánchez B;Sánchez-Vizcaíno JM;Noad R;Roy P
通讯作者：
Roy P
影响因子：
5.4
作者：
Boyce, Mark;Celma, Cristina C. P.;Roy, Polly
通讯作者：
Roy, Polly
影响因子：
5.4
作者：
Chauveau, Emilie;Doceul, Virginie;Vitour, Damien
通讯作者：
Vitour, Damien
影响因子：
3.3
作者：
Martinelle, Ludovic;Dal Pozzo, Fabiana;Saegerman, Claude
通讯作者：
Saegerman, Claude
DOI：
10.1073/pnas.192432299
发表时间：
2002-10-01
影响因子：
11.1
作者：
Beaton, AR;Rodriguez, J;Roy, P
通讯作者：
Roy, P