Utilization of Human Samples for Assessment of Mitochondrial Bioenergetics: Gold Standards, Limitations, and Future Perspectives.
Utilization of Human Samples for Assessment of Mitochondrial Bioenergetics: Gold Standards, Limitations, and Future Perspectives.
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DOI：
10.3390/life11090949
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发表时间：
2021-09-10
期刊：
影响因子：
--
通讯作者：
Stiles L
中科院分区：
文献类型：
--
作者：
Acin-Perez R;Benincá C;Shabane B;Shirihai OS;Stiles L
线粒体生物能功能是健康和疾病中细胞代谢的核心组成部分。线粒体氧化磷酸化对于维持能量稳态至关重要，线粒体功能受损是代谢疾病和衰老的发生和进展的基础。然而，由于收集的样本大小的限制，在人类样本中测量线粒体生物能功能可能具有挑战性。此外，从人类群体中收集样本通常分散在多天和多地点，这使得即时样本处理和生物能学分析具有挑战性。因此，样本的选择和测试的选择应该仔细考虑。基础研究、临床试验和线粒体疾病诊断主要依赖于骨骼肌样本。然而，获得骨骼肌活检需要适当的临床环境和专业人员，这使得骨骼肌不太适合某些研究。循环白细胞和血小板为线粒体生物能学研究提供了一种有前途的替代活检的原代组织。冷冻呼吸测量方案的最新进展与微创和无创样本的利用相结合，可能为未来的人类线粒体研究提供希望。在这里，我们回顾了常用于测量线粒体生物能学的人体样本，重点关注每个样本的优点和局限性。
Mitochondrial bioenergetic function is a central component of cellular metabolism in health and disease. Mitochondrial oxidative phosphorylation is critical for maintaining energetic homeostasis, and impairment of mitochondrial function underlies the development and progression of metabolic diseases and aging. However, measurement of mitochondrial bioenergetic function can be challenging in human samples due to limitations in the size of the collected sample. Furthermore, the collection of samples from human cohorts is often spread over multiple days and locations, which makes immediate sample processing and bioenergetics analysis challenging. Therefore, sample selection and choice of tests should be carefully considered. Basic research, clinical trials, and mitochondrial disease diagnosis rely primarily on skeletal muscle samples. However, obtaining skeletal muscle biopsies requires an appropriate clinical setting and specialized personnel, making skeletal muscle a less suitable tissue for certain research studies. Circulating white blood cells and platelets offer a promising primary tissue alternative to biopsies for the study of mitochondrial bioenergetics. Recent advances in frozen respirometry protocols combined with the utilization of minimally invasive and non-invasive samples may provide promise for future mitochondrial research studies in humans. Here we review the human samples commonly used for the measurement of mitochondrial bioenergetics with a focus on the advantages and limitations of each sample.
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影响因子：
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