Identification of a new Bombyx mori nucleopolyhedrovirus and analysis of its bro gene family

Identification of a new Bombyx mori nucleopolyhedrovirus and analysis of its bro gene family
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一株新的家蚕核多角体病毒的鉴定及其bro基因家族分析

DOI:
10.1007/s11262-012-0721-1
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发表时间:
2012-02
期刊:
影响因子:
1.6
通讯作者:
Xu, Jia-Ping
Xu, Jia-Ping
中科院分区:
医学4区
文献类型:
--
作者:
Zhou, Jing-Bo;Li, Xing-Qi;De-Eknamkul, Wanchai;Suraporn, Siripuk;Xu, Jia-Ping

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高致病性家蚕核多角体病毒(Bombyx mori nuclear polyhedrovirus, BmNPV)对许多国家的蚕桑养殖业造成了严重的危害,各种家蚕核多角体病毒毒株的致病性与其地理进化之间的关系一直是我们关注的话题。在这项研究中,我们根据其保守基因p10、p35、polh、egt和vp39的序列从泰国分离到了一株新的BmNPV- thai。泰国BmNPV病毒似乎具有杆状病毒重复的ORF (bro)基因,与中国(GD, CQ1),日本(T3)和法国(SC7)的其他四种知名BmNPV病毒株不同;它只有兄弟-a,兄弟-c和兄弟-d,但没有兄弟-b和兄弟-e基因。这些bro基因仅定位于两个高度同源的亚群中,其编码的bro蛋白主要在其n端氨基酸残基上存在差异。
The highly pathogenic Bombyx mori nucleopolyhedrovirus (BmNPV) has caused severe damages to sericulture in many countries, and the relationship between the pathogenicity of various BmNPV strains and their geographical evolution has been the topic of our interest. In this study, we isolated a new BmNPV strain from Thailand (BmNPV-Thai), based on the sequences of its conservative genes p10, p35, polh, egt and vp39. The BmNPV-Thai appears to have baculovirus repeated ORF (bro) genes different from four other well-known BmNPV strains of China (GD, CQ1), Japan (T3), and France (SC7); It only has bro-a, bro-c, and bro-d, but not bro-b and bro-e genes. These bro genes are localized only in the two subgroups highly homologous to their counterparts and their encoded BRO proteins differ mainly at their N-terminal amino acid residues.
DOI: 10.1007/bf02728943
发表时间: 2000-08
影响因子: 1.5
作者:
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