Examination of gene expression in saliva samples from COVID-19 patients to study the host defense response against SARS-CoV-2 in the oral cavity.
Examination of gene expression in saliva samples from COVID-19 patients to study the host defense response against SARS-CoV-2 in the oral cavity.
复制标题
检查来自Covid-19患者的唾液样本中的基因表达，以研究对口腔中SARS-COV-2的宿主防御反应。
DOI：
10.1111/omi.12327
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发表时间：
2021-04
影响因子：
3.7
通讯作者：
Glover SC
中科院分区：
文献类型：
--
作者：
Diamond G;Figgins EL;Robinson T;Senitko M;Abraham GE;Williams HB;Sloan M;Owings A;Laird H;Pride Y;Wilson KJ;Hasan M;Parker A;Glover SC
严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 感染会引起 2019 冠状病毒病 (COVID-19)，该病会出现发烧、喉咙痛、咳嗽和充血等呼吸道症状，表明口腔和鼻咽 (ONP) 感染是其发病机制中的必然步骤。与初始感染中的这种作用一致，口腔和鼻咽上皮细胞表达 SARS-CoV-2 刺突蛋白受体、血管紧张素转换酶 2 (ACE2)（Sakaguchi 等人，2020），基于 RT-PCR 的测试可以常规检测鼻和颊组织内的 SARS-CoV-2 RNA。口腔上皮细胞中的局部先天性和适应性免疫反应几乎肯定参与了针对 SARS-CoV-2 感染的防御，但人们对这些防御知之甚少。在阐明口腔抗病毒防御的初步方法中，我们确定了口腔上皮细胞中表达的抗病毒 I 型干扰素 (IFN) 和 IFN 刺激基因 (ISG) 的表达 (Brice 等，2019)。此外，我们还证明了维生素 D 介导的口腔上皮细胞内抗菌素的诱导作用（McMahon 等人，2011；Menzel 等人，2019）。 Cathelicidin 是一种宿主防御肽，可通过膜破坏使另一种有包膜的口腔传播病毒失活（Brice 等人，2018）。然而，我们的
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19), which presents with respiratory symptoms including fever, sore throat, cough, and congestion, suggesting that infection of the oral cavity and nasopharynx (ONP) is an obligate step in its pathogenesis. Consistent with this role in the initial infection, oral and nasopharyngeal epithelial cells express the SARS-CoV-2 spike protein receptor, angiotensin-converting enzyme 2 (ACE2)(Sakaguchi et al., 2020), and RT-PCR based testing can routinely detect SARS-CoV-2 RNA within both the nasal and buccal tissues. Local innate and adaptive immune responses in the oral epithelia are almost certainly involved in the defense against SARS-CoV-2 infection, yet little is known about these defenses.In an initial approach to elucidate the antiviral defense in the oral cavity, we identified the expression of the antiviral type I interferon (IFN) and IFN-stimulated genes (ISGs) expressed in oral epithelial cells (Brice et al., 2019). In addition, we demonstrated the vitamin D-mediated induction of cathelicidin (McMahon et al., 2011; Menzel et al., 2019) in the oral epithelium. Cathelicidin is a host defense peptide that inactivates another enveloped, orally transmitted virus through membrane disruption (Brice et al., 2018). However, our
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影响因子：
5.6
作者：
Sakaguchi W;Kubota N;Shimizu T;Saruta J;Fuchida S;Kawata A;Yamamoto Y;Sugimoto M;Yakeishi M;Tsukinoki K
通讯作者：
Tsukinoki K
影响因子：
3.5
作者：
Menzel, Lorenzo P.;Ruddick, Willam;Diamond, Gill
通讯作者：
Diamond, Gill
影响因子：
82.9
作者：
Kuba K;Imai Y;Rao S;Gao H;Guo F;Guan B;Huan Y;Yang P;Zhang Y;Deng W;Bao L;Zhang B;Liu G;Wang Z;Chappell M;Liu Y;Zheng D;Leibbrandt A;Wada T;Slutsky AS;Liu D;Qin C;Jiang C;Penninger JM
通讯作者：
Penninger JM
影响因子：
3.7
作者：
Brice, D. C.;Figgins, E.;Diamond, G.
通讯作者：
Diamond, G.
影响因子：
3.1
作者：
McMahon, Laura;Schwartz, Kyell;Diamond, Gill
通讯作者：
Diamond, Gill