Investigation on the Interactions of NiCR and NiCR-2H with DNA.
Investigation on the Interactions of NiCR and NiCR-2H with DNA.
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DOI：
10.1155/2010/619436
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发表时间：
2010
影响因子：
3.8
通讯作者：
Xue L
中科院分区：
文献类型：
--
作者：
Chitranshi P;Chen CN;Jones PR;Faridi JS;Xue L
We report here a biophysical and biochemical approach to determine the differences in interactions of NiCR and NiCR-2H with DNA. Our goal is to determine whether such interactions are responsible for the recently observed differences in their cytotoxicity toward MCF-7 cancer cells. Viscosity measurement and fluorescence displacement titration indicated that both NiCR and NiCR-2H bind weakly to duplex DNA in the grooves. The coordination of NiCR-2H with the N-7 of 2′-deoxyguanosine 5′-monophosphate (5′-dGMP) is stronger than that of NiCR as determined by 1H NMR. NiCR-2H, like NiCR, can selectively oxidize guanines present in distinctive DNA structures (e.g., bulges), and notably, NiCR-2H oxidizes guanines more efficiently than NiCR. In addition, UV and 1H NMR studies revealed that NiCR is oxidized into NiCR-2H in the presence of KHSO5 at low molar ratios with respect to NiCR (≤4).
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影响因子：
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作者：
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影响因子：
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