Repetitive Electroacupuncture Attenuates Cold-Induced Hypertension through Enkephalin in the Rostral Ventral Lateral Medulla.
Repetitive Electroacupuncture Attenuates Cold-Induced Hypertension through Enkephalin in the Rostral Ventral Lateral Medulla.
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DOI：
10.1038/srep35791
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发表时间：
2016-10-24
影响因子：
4.6
通讯作者：
Longhurst JC
中科院分区：
文献类型：
--
作者：
Li M;Tjen-A-Looi SC;Guo ZL;Longhurst JC
针灸可以降低高血压患者的血压，但其作用机制尚不清楚。为了模拟临床研究，我们在未麻醉的大鼠中进行电针（EA），这些大鼠通过6周的冷暴露（6 °C）诱导冷诱导高血压（CIH）。电针（0.1 ~ 0.4mA，2 Hz）于腓深神经上ST 36 -37穴位，每周2次，每次30 min，共5周。假电针除不电刺激外，其余操作同电针。18只CIH大鼠经6次电针治疗后血压下降，72小时后血压仍保持在较低水平，但假电针组（n = 12）和未治疗组（n = 6）CIH大鼠血压无明显变化。电针后72小时，大鼠延髓头端腹外侧区（rVLM）前脑啡肽原mRNA水平（n = 9）明显高于假电针组（n = 6）、CIH组（n = 6）和正常对照组（n = 6）。在电针治疗的CIH大鼠rVLM内微量注射δ阿片受体拮抗剂ICI 174，864，可部分逆转电针对血压升高的作用（n = 4）。用δ-阿片受体激动剂DADLE刺激假电针治疗的CIH大鼠的rVLM，降低血压（n = 6）。这些数据表明，增加脑啡肽在rVLM由重复EA诱导有助于EA的降压作用。
Acupuncture lowers blood pressure (BP) in hypertension, but mechanisms underlying its action are unclear. To simulate clinical studies, we performed electroacupuncture (EA) in unanesthetized rats with cold-induced hypertension (CIH) induced by six weeks of cold exposure (6 °C). EA (0.1 – 0.4 mA, 2 Hz) was applied at ST36-37 acupoints overlying the deep peroneal nerve for 30 min twice weekly for five weeks while sham-EA was conducted with the same procedures as EA except for no electrical stimulation. Elevated BP was reduced after six sessions of EA treatment and remained low 72 hrs after EA in 18 CIH rats, but not in sham-EA (n = 12) and untreated (n = 6) CIH ones. The mRNA level of preproenkephalin in the rostral ventrolateral medulla (rVLM) 72 hr after EA was increased (n = 9), compared to the sham-EA (n = 6), untreated CIH rats (n = 6) and normotensive control animals (n = 6). Microinjection of ICI 174,864, a δ-opioid receptor antagonist, into the rVLM of EA-treated CIH rats partially reversed EA’s effect on elevated BP (n = 4). Stimulation of rVLM of CIH rats treated with sham-EA using a δ-opioid agonist, DADLE, decreased BP (n = 6). These data suggest that increased enkephalin in the rVLM induced by repetitive EA contributes to BP lowering action of EA.
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影响因子：
7.4
作者：
de Hoyos, JAG;Martín, MDA;Moreno, MJG
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发表时间：
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影响因子：
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作者：
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通讯作者：
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