Cold-induced [Ca2+]cyt elevations function to support osmoregulation in marine diatoms.
Cold-induced [Ca2+]cyt elevations function to support osmoregulation in marine diatoms.
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DOI：
10.1093/plphys/kiac324
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发表时间：
2022-09-28
期刊：
影响因子：
7.4
通讯作者：
Brownlee, Colin
中科院分区：
文献类型：
--
作者：
Kleiner, Friedrich H.;Helliwell, Katherine E.;Chrachri, Abdul;Hopes, Amanda;Parry-Wilson, Hannah;Gaikwad, Trupti;Mieszkowska, Nova;Mock, Thomas;Wheeler, Glen L.;Brownlee, Colin
硅藻是一组微藻，是一系列开阔海洋、淡水和潮间带环境中重要的初级生产者。后者可以经历大量的长期和短期的温度变化，从季节变化到潮汐浸没和退潮引起的快速温度变化。由于温度是硅藻分布的主要决定因素，硅藻的温度感觉和反应机制可能在其生态成功中起重要作用。我们研究了硅藻用来感知温度快速变化的机制，比如在潮间带经历的那些变化。我们发现硅藻褐指藻（Phaeodactylum tricornutum）和假海硅藻（Thalassiosira pseudonana）在快速冷却下表现出短暂的胞浆Ca2+ ([Ca2+]cyt)升高，与植物和动物细胞中观察到的相似。然而，没有观察到[Ca2+]cyt升高对快速变暖的反应。冷诱导的[Ca2+]cyt升高的动力学和幅度与温度降低的速率相对应。我们没有发现[Ca2+]细胞升高在增强耐寒性中的作用，但表明冷休克诱导Ca2+依赖的K+外溢，并在同时发生的低渗透休克期间降低三角弓形虫的死亡率。由于潮间带硅藻物种可能在潮汐周期中经常同时遇到冷冲击和低渗透冲击，我们提出冷诱导的Ca2+信号通路与渗透信号通路相互作用，以帮助调节细胞体积。我们的研究结果为硅藻温度感知的本质提供了见解，并强调了信号通路之间的串扰可能在它们对多个同时发生的应激源的细胞反应中起重要作用。低温激活海洋硅藻钙信号通路，提高其在低渗透胁迫下的生存能力。
Diatoms are a group of microalgae that are important primary producers in a range of open ocean, freshwater, and intertidal environments. The latter can experience substantial long- and short-term variability in temperature, from seasonal variations to rapid temperature shifts caused by tidal immersion and emersion. As temperature is a major determinant in the distribution of diatom species, their temperature sensory and response mechanisms likely have important roles in their ecological success. We examined the mechanisms diatoms use to sense rapid changes in temperature, such as those experienced in the intertidal zone. We found that the diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana exhibit a transient cytosolic Ca2+ ([Ca2+]cyt) elevation in response to rapid cooling, similar to those observed in plant and animal cells. However, [Ca2+]cyt elevations were not observed in response to rapid warming. The kinetics and magnitude of cold-induced [Ca2+]cyt elevations corresponded with the rate of temperature decrease. We did not find a role for the [Ca2+]cyt elevations in enhancing cold tolerance but showed that cold shock induces a Ca2+-dependent K+ efflux and reduces mortality of P. tricornutum during a simultaneous hypo-osmotic shock. As intertidal diatom species may routinely encounter simultaneous cold and hypo-osmotic shocks during tidal cycles, we propose that cold-induced Ca2+ signaling interacts with osmotic signaling pathways to aid in the regulation of cell volume. Our findings provide insight into the nature of temperature perception in diatoms and highlight that cross-talk between signaling pathways may play an important role in their cellular responses to multiple simultaneous stressors. A calcium signaling pathway in marine diatoms is activated by cold temperature and enhances survival during simultaneous hypo-osmotic stress.
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