Antenna organization in green photosynthetic bacteria. 1. Oligomeric bacteriochlorophyll c as a model for the 740 nm absorbing bacteriochlorophyll c in Chloroflexus aurantiacus chlorosomes.
Antenna organization in green photosynthetic bacteria. 1. Oligomeric bacteriochlorophyll c as a model for the 740 nm absorbing bacteriochlorophyll c in Chloroflexus aurantiacus chlorosomes.
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绿色光合细菌的触角组织。
DOI：
10.1021/bi00400a023
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Blankenship,RE
中科院分区：
文献类型：
--
作者：
Brune,DC;Nozawa,T;Blankenship,RE
Department of Chemistry，Arizona州立大学，滕佩，Arizona 85287-1604接收于1987年5月15日;修订的Mandarin pt接收于1987年8月25日摘要：细菌叶绿素（BChl）c是从Chloroflexus aurantiacus中提取的，并通过反相高压液相色谱法纯化。该色素由同系物的复杂混合物组成，其主要成分是4-乙基-5-甲基细菌叶绿素c硬脂酸酯。与先前表征的BChls c不同，C.橙花素是在2A手性中心具有不同构型的非对映异构体的外消旋混合物。用己烷稀释BChl c的浓缩二氯甲烷溶液产生在740-742和460-462 nm处具有最大吸收的低聚物。这两个低聚物的吸收光谱和荧光发射光谱（最大值在750 nm处）密切匹配的BChlc在叶绿体。进一步支持这一模型来自醇的能力，它通过连接中心Mg原子破坏BChl c低聚物，当以低浓度加入时，将BChl c在叶绿体中转化为单体形式。BChlc寡聚体在740 nm处的荧光寿命约为80 ps。虽然激子淬灭可能是异常快，在体外BChl c寡聚体，因为它的大尺寸和/或微量杂质的存在下，这一结果表明，能量转移从BChl c天线在叶绿体必须非常快，如果它是有效的。
Department of Chemistry, Arizona State University, Tempe, Arizona 85287-1604 Received May 15, 1987; Revised Manuscript Received August 25, 1987 abstract: Bacteriochlorophyll (BChl) c was extracted from Chloroflexus aurantiacus and purified by reverse-phase high-pressure liquid chromatography. This pigment consists of a complex mixture of homologues, the major component of which is 4-ethyl-5-methylbacteriochlorophyll c stearyl ester. Unlike previously characterized BChls c, the pigment from C. aurantiacus is a racemic mixture of diastereoisomers with different configurations at the 2a chiral center. Diluting a concentrated methylene chloride solution of BChl c with hexane produces an oligomer with absorption maxima at 740-742 and at 460-462 nm. Both the absorption spectrum and the fluorescence emission spectrum (maximum at 750 nm) of this oligomer closely match those of BChl c in chlorosomes. Further support for this model comes from the ability of alcohols, which disruptBChl c oligomers by ligating the central Mg atom, to convert BChl c in chlorosomes to a monomeric form when added in low concentrations. The lifetimeof fluorescence from the 740 nm absorbing BChl c oligomer is about 80 ps. Although excitonquenching might be unusually fast in the in vitro BChl c oligomer because of its large size and/or the presence of minor impurities, this result suggests that energy transferfrom the BChl c antenna in chlorosomes must be very fast if it is to be efficient.
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DOI：
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