Notch4 Signaling Pathway of Endothelial Progenitor Cells in a Kawasaki Disease Model Induced by Lactobacillus casei Cell Wall Extract
Notch4 Signaling Pathway of Endothelial Progenitor Cells in a Kawasaki Disease Model Induced by Lactobacillus casei Cell Wall Extract
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干酪乳杆菌细胞壁提取物诱导的川崎病模型中内皮祖细胞的Notch4信号通路
DOI：
10.1159/000449061
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Gong F
中科院分区：
文献类型：
--
作者：
Wang W;Xie C;Zhang Y;Gong F
内皮祖细胞（EPCs）的Notch 4信号通路可能在川崎病（KD）发病中起重要作用。我们研究了干酪乳杆菌细胞壁提取物诱导的KD模型中骨髓（BM）EPCs的增殖、粘附、迁移、血管生成以及Notch 4、重组信号结合蛋白-J κ（RBP-Jκ）、P-选择素和血管细胞粘附分子-1（VCAM-1）的表达水平。在KD模型中，BM EPCs的数量显著减少。在KD模型中EPC表面上的Notch 4表达水平高于对照。在KD模型中，BM EPCs的增殖、粘附、迁移和血管生成特性以及双重免疫荧光结合率显著受损。在KD模型中Notch 4和P-选择素mRNA的水平在第3天低于对照。在KD模型中，RBP-Jκ mRNA水平在第3天和第7天低于对照组。RBP-Jκ和血管内皮生长因子受体2蛋白水平在早期下降。结论：KD模型大鼠骨髓EPC功能和生物活性受损，Notch 4信号通路与KD相关。
The Notch4 signaling pathway of endothelial progenitor cells (EPCs) may play a crucial role in Kawasaki disease (KD). We investigated the proliferation, adhesion, migration, angiogenesis, and expression levels of Notch4, recombination signal-binding protein-Jκ (RBP-Jκ), P-selectin, and vascular cell adhesion molecule-1 (VCAM-1) of bone marrow (BM) EPCs in a KD model induced by Lactobacillus casei cell wall extract. The numbers of BM EPCs decreased significantly in the KD models. The Notch4 expression level on the EPC surface was higher in the KD models than in the controls. The proliferative, adhesive, migratory, and angiogenic properties, and double immunofluorescence-binding rate of BM EPCs were significantly impaired in the KD models. The levels of Notch4 and P-selectin mRNA were lower in the KD models than in the controls on day 3. The RBP-Jκ mRNA levels were lower in the KD models than in the controls on days 3 and 7. The levels of RBP-Jκ and vascular endothelial growth factor receptor-2 proteins decreased in the early stage. In conclusion, the BM EPC functions and bioactivities in the KD models were impaired, and the Notch4 signaling pathway is associated with KD.
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影响因子：
5.2
作者：
Wary, Kishore K.;Vogel, Stephen M.;Garrean, Sean;Zhao, Yidan D.;Malik, Asrar B.
通讯作者：
Malik, Asrar B.
Notch-RBP-J 信号通过动态调节小鼠 CXCR4 表达来调节内皮祖细胞的动员和功能
影响因子：
3.7
作者：
Wang L;Wang YC;Hu XB;Zhang BF;Dou GR;He F;Gao F;Feng F;Liang YM;Dou KF;Han H
通讯作者：
Han H
影响因子：
4.4
作者：
Duong, TT;Silverman, ED;Yeung, RSM
通讯作者：
Yeung, RSM
影响因子：
4.7
作者：
Nakamura Y;Yashiro M;Uehara R;Sadakane A;Chihara I;Aoyama Y;Kotani K;Yanagawa H
通讯作者：
Yanagawa H
DOI：
10.1073/pnas.0611177104
发表时间：
2007-02-27
影响因子：
11.1
作者：
Suchting, Steven;Freitas, Catarina;Eichmann, Anne
通讯作者：
Eichmann, Anne