The differential expression pattern of the BMI-1, SALL4 and ABCA3 genes in myeloid leukemia.
The differential expression pattern of the BMI-1, SALL4 and ABCA3 genes in myeloid leukemia.
复制标题
粒细胞白血病中BMI-1、SALL4和ABCA3基因的差异表达模式
DOI：
10.1186/1475-2867-12-42
复制
发表时间：
2012-10-15
影响因子：
5.8
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Shen Q;Liu S;Hu J;Chen S;Yang L;Li B;Wu X;Ma Y;Yang J;Ma Y;Li Y
背景和方法
目的：研究髓系白血病患者和化疗后完全缓解(CR)患者SALL4、Bmi-1和ABCA3基因的表达特点。采用实时定量聚合酶链式反应检测24例AML、8例AML-CR、13例CML慢性期(CML-CP)、12例CML急变期(CML-BC)、13例CML-CR和11例健康人(HI)外周血单个核细胞中这些基因的表达水平。
结果
与HI组相比，AML、CML-CP和CML-BC组BMI-1基因高表达，而获得CR的患者BMI-1表达水平较低。CML-CP组和CML-CR组SALL4表达均低于HI组，而CML-BC组SALL4表达水平高于CML-CP组和CML-CR组，且差异有统计学意义。此外，在大多数组的样本中，SALL4和BMI-1基因的表达呈正相关。AML组和CML-BC组ABCA3表达水平与HI组比较差异无统计学意义。有趣的是，与HI组相比，CML-CP、AML-CR和CML-CR组ABCA3的表达水平显著降低。此外，所有CR组的ABCA3表达水平均低于其对应的组。
结论
这些结果描述了SALL4、ABCA3和BMI-1在髓系白血病不同阶段的表达变化，可能与不同疾病的发生发展有关。在大多数髓系白血病患者中，SALL4的表达与BMI-1密切相关，这为SALL4与BMI-1在白血病发生中的作用提供了潜在的联系。
Background and methods
In order to characterize the expression pattern of SALL4, BMI-1 and ABCA3 genes in patients with myeloid leukemia and those who achieved complete remission (CR) after chemotherapy. Real-time PCR was used to determine the expression level of these genes in peripheral blood mononuclear cells from 24 patients with AML, eight patients with AML-CR, 13 patients with CML in the chronic phase (CML-CP), 12 patients with CML in blast crisis (CML-BC), 13 patients with CML-CR and 11 healthy individuals (HI).
Results
Overexpression of the BMI-1 gene was found in the AML, CML-CP and CML-BC groups as compared with HI group, while the BMI-1 expression level was lower in patients who achieved CR. In contrast, significantly increased SALL4 expression was only found in AML group, additionally, SALL4 expression was lower in the CML-CP and CML-CR groups compared with the HI group, while the SALL4 expression level in the CML-BC group was higher and significantly greater than that in the CML-CP and CML-CR groups. Moreover, a positive correlation between the expression of SALL4 and BMI-1 genes was found in samples from most groups. There was no significant difference of ABCA3 expression level in AML and CML-BC group in comparison with HI group. Interestingly, the ABCA3 expression level was significantly decreased in the CML-CP, AML-CR and CML-CR in comparison with the HI group. Moreover, the ABCA3 expression level in all of the CR groups was lower than that in their corresponding groups.
Conclusions
These results describe the altered SALL4, ABCA3 and BMI-1 expression pattern in different phases of myeloid leukemia, which may relate to the development and progression to different diseases. SALL4 expression was strongly correlated with BMI-1 in most of the myeloid leukemia patient groups, providing a potential link between SALL4 and BMI-1 in leukemogenesis.
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影响因子：
28.5
作者：
Braoudaki M;Papathanassiou C;Katsibardi K;Tourkadoni N;Karamolegou K;Tzortzatou-Stathopoulou F
通讯作者：
Tzortzatou-Stathopoulou F
影响因子：
16.8
作者：
Raaphorst, FM
通讯作者：
Raaphorst, FM
影响因子：
9.8
作者：
Al-Baradie, R;Yamada, K;Engle, EC
通讯作者：
Engle, EC
影响因子：
3.7
作者：
Jeong HW;Cui W;Yang Y;Lu J;He J;Li A;Song D;Guo Y;Liu BH;Chai L
通讯作者：
Chai L
polycomb基因rae28是维持造血干细胞活性所必需的。
DOI：
10.1084/jem.20011911
发表时间：
2002-03-18
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Ohta H;Sawada A;Kim JY;Tokimasa S;Nishiguchi S;Humphries RK;Hara J;Takihara Y
通讯作者：
Takihara Y