Automated detection system of single nucleotide polymorphisms using two kinds of functional magnetic nanoparticles
Automated detection system of single nucleotide polymorphisms using two kinds of functional magnetic nanoparticles
复制标题
两种功能性磁性纳米粒子的单核苷酸多态性自动检测系统
DOI：
10.1016/j.apsusc.2008.06.117
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发表时间：
2008-11
影响因子：
6.7
通讯作者：
Li, S
中科院分区：
文献类型：
--
作者：
Deng, Y;He, NY;Wang, ZF;Shi, ZY;Li, ZY;Wang, H;Liu, HN;Li, S
Single nucleotide polymorphisms (SNPs) comprise the most abundant source of genetic variation in the human genome wide codominant SNPs identification. Therefore, large-scale codominant SNPs identification, especially for those associated with complex diseases, has induced the need for completely high-throughput and automated SNP genotyping method. Herein, we present an automated detection system of SNPs based on two kinds of functional magnetic nanoparticles (MNPs) and dual-color hybridization. The amido-modified MNPs (NH2-MNPs) modified with APTES were used for DNA extraction from whole blood directly by electrostatic reaction, and followed by PCR, was successfully performed. Furthermore, biotinylated PCR products were captured on the streptavidin-coated MNPs (SA–MNPs) and interrogated by hybridization with a pair of dual-color probes to determine SNP, then the genotype of each sample can be simultaneously identified by scanning the microarray printed with the denatured fluorescent probes. This system provided a rapid, sensitive and highly versatile automated procedure that will greatly facilitate the analysis of different known SNPs in human genome.
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影响因子：
12.6
作者：
T. Yoshino;Tsuyoshi Tanaka;H. Takeyama;T. Matsunaga
通讯作者：
T. Yoshino;Tsuyoshi Tanaka;H. Takeyama;T. Matsunaga
影响因子：
--
作者：
S. Harder;F. Feil;K. Knoll
通讯作者：
S. Harder;F. Feil;K. Knoll
影响因子：
--
作者：
Song Li;Meiju Ji;N. He;Zuhong Lu
通讯作者：
Song Li;Meiju Ji;N. He;Zuhong Lu
影响因子：
1.6
作者：
Shen, XC;Fang, XZ;Liang, H
通讯作者：
Liang, H
影响因子：
12.6
作者：
Lermo, A.;Campoy, S.;Pividori, M. I.
通讯作者：
Pividori, M. I.