Dynamics of circulating endothelial cells and endothelial progenitor cells in breast cancer patients receiving cytotoxic chemotherapy.
Dynamics of circulating endothelial cells and endothelial progenitor cells in breast cancer patients receiving cytotoxic chemotherapy.
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DOI：
10.1186/1471-2407-12-620
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发表时间：
2012-12-26
期刊：
影响因子：
3.8
通讯作者：
Cheng AL
中科院分区：
文献类型：
--
作者：
Kuo YH;Lin CH;Shau WY;Chen TJ;Yang SH;Huang SM;Hsu C;Lu YS;Cheng AL
作为血管生成的替代标志物，循环内皮细胞（CECs）和循环内皮祖细胞（cep）的丰度可能受到化疗的影响。我们研究了它们在连续化疗周期中的动态变化。我们采集了共接受56个疗程全身化疗的15例乳腺癌患者的血液样本，在化疗前7天，第一和第二化疗周期每周2次，随后化疗周期每周1次，用六色流式细胞术测量CECs、活CECs （V-CECs）和cep。从治疗第1天到化疗第1周，CEC、V-CEC和CEP水平均显著下降。化疗一周后，CEC和V-CEC水平恢复到与第1天相似的水平。化疗第一周后CEP水平仍明显降低，但逐渐回升至下一个疗程。化疗6个周期后，CEC和V-CEC细胞总数呈减少趋势，CEP细胞呈增加趋势。临床因素，包括肿瘤的存在、化疗方案和粒细胞集落刺激因子的使用，对这些结果没有显著影响。CEC和CEP计数在每个化疗过程中以及化疗周期后都是动态变化的，这为未来研究计划在抗血管生成联合化疗中使用CEC和CEP作为血管生成的替代标志物提供了背景数据。
The abundance of circulating endothelial cells (CECs) and circulating endothelial progenitor cells (CEPs), which serve as surrogate markers for angiogenesis, may be affected by chemotherapy. We studied their dynamic change during consecutive cycles of chemotherapy. We collected blood samples from 15 breast cancer patients, who received a total of 56 courses of systemic chemotherapy, and measured the CECs, viable CECs (V-CECs), and CEPs by six-color flow cytometry within the seven days prior to chemotherapy, twice a week during the first and second cycles of chemotherapy, and then once a week during the subsequent cycles. The CEC, V-CEC, and CEP levels all significantly decreased from day 1 of treatment to the first week of chemotherapy. After one week of chemotherapy, the CEC and V-CEC levels returned to a level similar to day 1. The CEP level remained significantly reduced after the first week of chemotherapy, but gradually rebounded until the next course of chemotherapy. After six cycles of chemotherapy, the total number of CEC and V-CEC cells trended toward a decrease and the CEP cells toward an increase. Clinical factors, including the existence of a tumor, chemotherapy regimens, and the use of granulocyte colony stimulating factor, did not significantly affect these results. The CEC and CEP counts change dynamically during each course of chemotherapy and after the chemotherapy cycles, providing background data for any future study planning to use CECs and CEPs as surrogate markers of angiogenesis in antiangiogenesis treatments combined with chemotherapy.
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影响因子：
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作者：
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