Permeabilized cell models for the study of granule transport in pigment cells.
Permeabilized cell models for the study of granule transport in pigment cells.
复制标题
用于研究色素细胞中颗粒运输的透化细胞模型。
DOI：
10.1111/j.1600-0749.1987.tb00391.x
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Manfred Schliwa
中科院分区：
文献类型：
--
作者：
Manfred Schliwa
在低等脊椎动物和一些无脊椎动物中，颜色的变化是由盘状或星状特化细胞（称为铬酸盐发光体）中数千个含色素囊泡的协调运动引起的。色素颗粒向细胞中心的向心运动，一个被称为聚集的过程，导致皮肤变亮，而它们在整个细胞体中或多或少的均匀分布或分散，根据色素的颜色使动物的体表着色。简单地操作介质将引发色素颗粒沿着径向路径的向心或离心（向内或向外，逆行或顺行）运动，速度高达8皮秒。事实上，所有的真核细胞都能在细胞质中快速、矢量地移动膜结合的细胞器，色素的运输只是其中一个不可否认的显著表现。这种基本细胞活动的其他例子包括快速轴突运输或某些原生动物（如异格虫和网黏虫）网状足网络中的颗粒运动。尽管经过了二十年的深入研究，细胞器运输的分子机制，特别是色素颗粒运输的分子机制，除了微管以某种方式参与的一般结论外，还没有得到研究人员的理解。这一概念是基于这样的发现，即微管分布在空间上与细胞器的途径相关，并且它们的解聚导致这些运动的停止。驱动细胞器运输的分子的身份仍然完全不清楚;事实上，即使是微管是否直接参与或仅作为微管独立运动（例如，肌动球蛋白）的行动指南的基本问题也无法得到令人满意的解决（最近的评论，请参阅Schliwa，1984;
Color change in lower vertebrates and some invertebrates is brought about by the coordinated movement of thousands of pigment-containing vesicles within discoid or stellate-shaped specialized cells termed chromate phores. The centripetal movement of pigment granules towards the cell center, a process referred to as aggregation, leads to lightening of the skin, whereas their more or less uniform distribution throughout the cell body, or dispersion, tints the body surface of the animal according to the color of the pigment. Simple manipulations of the medium bathing the chromatophores will elicit the centripetal or centrifugal (inward or outward, retrograde or anterograde) movement of pigment granules along radial pathways at rates of up to 8 pdsec. As such pigment transport is but one admittedly striking manifestation of the ability of virtually all eukaryotic cells to move membrane-bound organelles rapidly and vectorially about the cytoplasm. Other examples of this fundamental cellular activity include fast axonal transport or particle movements in reticulopodial networks of certain protozoans such as Allogromia and Reticulomyxa. Notwithstanding two decades of intense research, the molecular mechanisms of organelle transport in general and pigment granule transport in particular have eluded investigators, except for the general conclusion that microtubules are somehow involved. This notion was based on the findings that microtubule distribution correlates spatially with the pathways of organelles and that their depolymerization leads to cessation of these movements. The identity of the molecule (s) that drive organelle transport remained entirely unclear; in fact, even the basic question of whether microtubules are directly involved or merely serve as guidelines for the action of a microtubule-independent motor (eg, actomyosin) could not be resolved to everyone’s satisfaction (for recent reviews, see Schliwa, 1984;
色素细胞器易位的调节。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lynch,TJ;Taylor,JD;Tchen,TT
通讯作者：
Tchen,TT
色素细胞器易位的调节。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lynch,TJ;Wu,BY;Taylor,JD;Tchen,TT
通讯作者：
Tchen,TT
影响因子：
56.9
作者：
BRADY, ST;LASEK, RJ;ALLEN, RD
通讯作者：
ALLEN, RD