Development of non-destructive, non-contact single-cell based differential cell assay using on-chip microcultivation and optical tweezers
Development of non-destructive, non-contact single-cell based differential cell assay using on-chip microcultivation and optical tweezers
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使用片上微培养和光镊开发非破坏性、非接触式单细胞差异细胞测定
DOI：
10.1016/s0925-4005(03)00549-5
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发表时间：
2003
影响因子：
8.4
通讯作者：
K. Yasuda
中科院分区：
文献类型：
--
作者：
Y. Wakamoto;S. Umehara;K. Matsumura;I. Inoue;K. Yasuda
我们研究了基于芯片上微培养和光钳的非破坏性、非接触式单细胞差异细胞筛选方法。用于培养4个单细胞的4个房间的微室由5μm高的微结构组成，在玻片上覆盖半透膜，以在无污染的条件下培养这些细胞。微室中的细胞数量是通过使用光学镊子挑出多余的细胞来控制的。为了研究细胞在隔离条件下发生的实际变化，我们在四个微室中同时测量并比较了超过10代的四个细胞。为了检验这种方法的潜力，我们对大肠杆菌的单个细胞进行了超过10代的观察。这些观测结果表明，即使在分化时间和生长速度上存在较大的动态变化，它们仍保持着它们的平均值。四个细胞的同时观察也证实，细胞中的这些变异不是来自环境条件的时间进程变化，因为四个细胞中的这些变异不是同步的。这些结果表明，我们的方法可能用于细胞检测，以预测细胞的变异是否在允许的范围内，或者是由药物或其他环境影响引起的。
We have investigated non-destructive, non-contact single-cell based differential cell screening method using on-chip microcultivation and optical tweezers. The four-room microchamber for cultivating four single cells consists of 5μm high microstructure on the glass slide and is covered with semipermeable membrane to cultivate those cells under contamination-free condition. The number of cells in microchambers is controlled by using optical tweezers to pick out excess cells. For studying the actual changes occurring in cells under isolated condition, we measured and compared four cells in four microchambers simultaneously for more than 10 generations. To examine the potential of this method, we observed single cells of Escherichia coli for more than 10 generations. The results of these observations showed that they keep their mean values even though there exist large dynamic variations in interdivision time and growth speed. The simultaneous observations of four cells also confirmed that these variations in cells are not derived from the time-course changes of environmental conditions because those variations in four cells did not synchronized. The results suggested the potential use of our method for cell assay to predict whether the cells’ variation is in permissible range or is caused by drugs or other environmental effects.
影响因子：
64.8
作者：
ASHKIN, A;DZIEDZIC, JM;YAMANE, T
通讯作者：
YAMANE, T