Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
Urinary miR-29 correlates with albuminuria and carotid intima-media thickness in type 2 diabetes patients.
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2 型糖尿病患者的尿液 miR-29 与白蛋白尿和颈动脉内膜中层厚度相关。
DOI：
10.1371/journal.pone.0082607
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Lou T
中科院分区：
文献类型：
--
作者：
Peng H;Zhong M;Zhao W;Wang C;Zhang J;Liu X;Li Y;Paudel SD;Wang Q;Lou T
无细胞microRNA在体液中稳定而丰富地存在，新的证据表明无细胞microRNA是一种新型的非侵入性疾病生物标志物。miR-29的失调参与了糖尿病肾病和胰岛素抵抗的发病机制，因此可能与糖尿病血管并发症有关。因此，我们研究了尿miR-29作为2型糖尿病患者糖尿病肾病和动脉粥样硬化的生物标志物的可能性。采用TaqMan qRT-PCR方法检测83例2型糖尿病患者尿液中miR-29 a、miR-29 b和miR-29 c的表达水平，并在提取miRNAs前加入合成的cel-miR-39作为对照。采用尿白蛋白排泄率、尿白蛋白/肌酐比值、眼底镜检查、颈动脉超声检查评价糖尿病血管并发症。同时收集血糖、肾功能和血脂等实验室指标。与正常白蛋白尿患者（n= 41，年龄58.54± 14.40岁）相比，白蛋白尿患者（n= 42，年龄60.62± 12.00岁）显示出显著更高的糖尿病视网膜病变合并症（p =0.015）和更高的尿miR-29 a水平（p=0.035）。 两组之间的尿液miR-29 b（p = 0.148）或miR-29 c（p =0.321）水平无显著差异。 尿白蛋白排泄率与尿miR-29 a水平显著相关（r = 0.286，p = 0.016），而尿miR-29 b与颈动脉内膜中层厚度（cIMT）显著相关（r = 0.286，p = 0.046）。 在2型糖尿病患者中，尿miR-29 a与白蛋白尿相关，而尿miR-29 b与颈动脉内膜中层厚度（cIMT）相关。因此，它们有可能作为2型糖尿病肾病和动脉粥样硬化的替代生物标志物。
Cell-free microRNAs stably and abundantly exist in body fluids and emerging evidence suggests cell-free microRNAs as novel and non-invasive disease biomarker. Deregulation of miR-29 is involved in the pathogenesis of diabetic nephropathy and insulin resistance thus may be implicated in diabetic vascular complication. Therefore, we investigated the possibility of urinary miR-29 as biomarker for diabetic nephropathy and atherosclerosis in patients with type 2 diabetes. 83 patients with type 2 diabetes were enrolled in this study, miR-29a, miR-29b and miR-29c levels in urine supernatant was determined by TaqMan qRT-PCR, and a synthetic cel-miR-39 was added to the urine as a spike-in control before miRNAs extraction. Urinary albumin excretion rate and urine albumin/creatinine ratio, funduscopy and carotid ultrasound were used for evaluation of diabetic vascular complication. The laboratory parameters indicating blood glucose level, renal function and serum lipids were also collected. Patients with albuminuria (n = 42, age 60.62±12.00yrs) showed significantly higher comorbidity of diabetic retinopathy (p = 0.015) and higher levels of urinary miR-29a (p = 0.035) compared with those with normoalbuminuria (n = 41, age 58.54±14.40yrs). There was no significant difference in urinary miR-29b (p = 0.148) or miR-29c level (p = 0.321) between groups. Urinary albumin excretion rate significantly correlated with urinary miR-29a level (r = 0.286, p = 0.016), while urinary miR-29b significantly correlated with carotid intima-media thickness (cIMT) (r = 0.286, p = 0.046). Urinary miR-29a correlated with albuminuria while urinary miR-29b correlated with carotid intima-media thickness (cIMT) in patients with type 2 diabetes. Therefore, they may have the potential to serve as alternative biomarker for diabetic nephropathy and atherosclerosis in type 2 diabetes.
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影响因子：
3.4
作者：
Natarajan, Rama;Putta, Sumanth;Kato, Mitsuo
通讯作者：
Kato, Mitsuo
影响因子：
16.2
作者：
Chen YH;Chen HS;Tarng DC
通讯作者：
Tarng DC
影响因子：
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作者：
van Dam, EM;Govers, R;James, DE
通讯作者：
James, DE
DOI：
10.1152/ajprenal.00287.2012
发表时间：
2013-05-01
影响因子：
4.2
作者：
Fang, Yi;Yu, Xiaofang;Ding, Xiaoqiang
通讯作者：
Ding, Xiaoqiang
影响因子：
168.9
作者：
Ferrannini, Ele;Cushman, William C.
通讯作者：
Cushman, William C.