Effects of nitric oxide-releasing nonsteroidal anti-inflammatory drugs (NONO-NSAIDs) on melanoma cell adhesion.
Effects of nitric oxide-releasing nonsteroidal anti-inflammatory drugs (NONO-NSAIDs) on melanoma cell adhesion.
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DOI：
10.1016/j.taap.2012.07.029
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发表时间：
2012-10-15
影响因子：
3.8
通讯作者：
Wu, Shiyong
中科院分区：
文献类型：
--
作者：
Cheng, Huiwen;Mollica, Molly Y.;Lee, Shin Hee;Wang, Lei;Velazquez-Martinez, Carlos A.;Wu, Shiyong
近年来开发的一类新型一氧化氮(NO·)释放型非甾体抗炎药(NONO-NSAIDs)因其对心血管和胃肠系统的温和性质而显示出作为非甾体抗炎药替代品的潜力。由于一氧化氮在调节细胞黏附中起作用，我们评估了NONO-NSAIDs作为抗转移药物的潜在用途。在这方面，我们比较了NONO-阿司匹林和一种新型的NONO-NAPROXen，以及它们各自的亲本NSAIDs对人类黑色素瘤M624细胞的亲和力的影响。在流体和静态条件下，NONO-NSAID和FN分别使M624对血管细胞黏附分子-1(VCAM-1)和纤维连接蛋白(FN)的黏附能力降低20-30%和25-44%。β-1整合素与α-4整合素结合形成VLA4的配体VLA4(VLA4)。这些结果表明，NO·供体部分在减少VLA-4与其配体之间的黏附中起关键作用，而NSAID部分可以影响黑色素瘤细胞黏附的调节机制。
A new class of nitric oxide (NO•)-releasing nonsteroidal anti-inflammatory drugs (NONO-NSAIDs) were developed in recent years and have shown promising potential as NSAID substitutes due to their gentle nature on cardiovascular and gastrointestinal systems. Since nitric oxide plays a role in regulation of cell adhesion, we assessed the potential use of NONO-NSAIDs as anti-metastasis drugs. In this regard, we compared the effects of NONO-aspirin and a novel NONO-naproxen, to those exerted by their respective parent NSAIDs on avidities of human melanoma M624 cells. Both NONO-NSAIDs, but not the corresponding parent NSAIDs, reduced M624 adhesion on vascular cellular adhesion molecule-1 (VCAM-1) by 20-30% and fibronectin by 25-44% under fluid flow conditions and static conditions, respectively. Only NONO-naproxen reduced (~56%) the activity of β1 integrin, which binds to α4 integrin to form very late antigen-4 (VLA-4), the ligand of VCAM-1. These results indicate that the diazeniumdiolate (NO•)-donor moiety is critical for reducing the adhesion between VLA-4 and its ligands, while the NSAID moiety can impact the regulation mechanism of melanoma cell adhesion.
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诱导型一氧化氮合酶（INOS）表达可能预测人黑色素瘤中的远处转移。
影响因子：
8.8
作者：
Tschugguel, W;Pustelnik, T;Lass, H;Mildner, M;Weninger, W;Schneeberger, C;Jansen, B;Tschachler, E;Waldhor, T;Huber, J C;Pehamberger, H
通讯作者：
Pehamberger, H
影响因子：
7.3
作者：
Velázquez, C;Rao, PNP;Knaus, EE
通讯作者：
Knaus, EE
影响因子：
3.5
作者：
Velazquez, Carlos A.;Rao, P. N. Praveen;Knaus, Edward E.
通讯作者：
Knaus, Edward E.
影响因子：
11.2
作者：
Lahdenranta J;Hagendoorn J;Padera TP;Hoshida T;Nelson G;Kashiwagi S;Jain RK;Fukumura D
通讯作者：
Fukumura D
影响因子：
4.3
作者：
Baritaki, Stavroula;Huerta-Yepez, Sara;Bonavida, Benjamin
通讯作者：
Bonavida, Benjamin