Fit-Seq2.0: An Improved Software for High-Throughput Fitness Measurements Using Pooled Competition Assays.

Fit-Seq2.0: An Improved Software for High-Throughput Fitness Measurements Using Pooled Competition Assays.
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DOI:
10.1007/s00239-023-10098-0
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发表时间:
2023-06
影响因子:
3.9
通讯作者:
--
中科院分区:
生物学3区
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--
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基因型的适应度被定义为其一生的繁殖成功率,适应度本身是一种复合性状,可能依赖于许多潜在的表型。测量适应度对于理解不同细胞成分的改变如何影响细胞的繁殖能力是很重要的。在这里,我们描述了一种改进的方法,用Python实现,通过池竞争分析来估计高吞吐量的适应度。
The fitness of a genotype is defined as its lifetime reproductive success, with fitness itself being a composite trait likely dependent on many underlying phenotypes. Measuring fitness is important for understanding how alteration of different cellular components affects a cell’s ability to reproduce. Here, we describe an improved approach, implemented in Python, for estimating fitness in high throughput via pooled competition assays.
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