Modulation of specific protein expression in teratocarcinoma cell aggregates by antibodies affecting cell-cell interactions.
Modulation of specific protein expression in teratocarcinoma cell aggregates by antibodies affecting cell-cell interactions.
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通过影响细胞间相互作用的抗体调节畸胎癌细胞聚集体中的特异性蛋白质表达。
DOI：
10.1016/0012-1606(85)90429-4
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发表时间：
1985
影响因子：
2.7
通讯作者：
M. Buc
中科院分区：
文献类型：
--
作者：
L. Maillet;M. Buc
在胚胎癌（EC） PSMB的胚状体形成和分化过程中，我们利用解压缩Fab作为工具来改变细胞间的相互作用。使用了两种Fab：仅识别黏附糖蛋白uvomorulin的anti-uvomorulin Fab和识别包括uvomorulin在内的一系列分子的anti-EC血清Fab。这两种类型的Fab都导致分解和松散聚集体的形成，并伴有细胞外基质（ECM）成分的异常沉积。抗uvomorulin Fab对胚状体分化模式无影响。相反，Fab抗ec诱导聚集体中的大多数细胞分化为内胚层样表型。这种效果只在抗ec Fab加入到聚集体中而不是单层培养物中观察到。
Decompacting Fab were used as tools to modify cell interactions during embryoid body formation and differentiation of the embryonal carcinoma (EC) PSMB. Two kinds of Fab were used: anti-uvomorulin Fab which recognize only the adhesion glycoprotein uvomorulin and Fab from an anti-EC serum which recognizes a series of molecules including uvomorulin. Both types of Fab led to decompaction and formation of loose aggregates with abnormal deposition of extracellular matrix (ECM) components. Anti-uvomorulin Fab did not affect the pattern of embryoid body differentiation. In contrast Fab anti-EC induced a differentiation of most of the cells in the aggregate into an endoderm-like phenotype. This effect was observed only when anti-EC Fab were added to aggregates and not to monolayer cultures.
DOI：
--
发表时间：
1978-02
期刊：
Journal of embryology and experimental morphology
影响因子：
--
作者：
By M. Dziadek;E. Adamson
通讯作者：
By M. Dziadek;E. Adamson