Random amplified polymorphic DNA reveals that TiO2 nanoparticles are genotoxic to Cucurbita pepo

Random amplified polymorphic DNA reveals that TiO2 nanoparticles are genotoxic to Cucurbita pepo
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随机扩增多态性 DNA 揭示 TiO2 纳米颗粒对葫芦具有遗传毒性

DOI:
10.1631/jzus.a1400159
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发表时间:
2014
期刊:
Journal of Zhejiang University SCIENCE A
影响因子:
--
通讯作者:
J. Gardea
J. Gardea
中科院分区:
--
文献类型:
--
作者:
Fabiola Moreno;V. Gant;Kyle L. Johnson;J. Peralta;J. Gardea

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Titanium dioxide nanoparticles (TiO2 NPs) are used in cosmetics, sunscreens, paints, and toothpaste, among other applications. These NPs are very stable and can be transported and dispersed in wastewater and biosolids. Animal species have shown negative reactions to TiO2 NPs. However, little is known about their toxicity in plants, specifically the possibility of genotoxic effects. In this study, we used a random amplified polymorphic DNA (RAPD) technique to study the genotoxic effects of TiO2 NPs on hydroponically cultivated zucchini (Cucurbita pepo) plants. Seeds were allowed to germinate for 7 d and plants were selected at random for individual and population studies. Four plants were selected for the individual study and 18 for the population study. RAPD profiles of TiO2 NPs treated plants showed differences in band intensity, loss of bands, or appearance of new bands, compared to untreated plants. To the authors’ knowledge, this is the first report of the genotoxic potential of TiO2 NPs in zucchini.概要研究目的二氧化钛 (TiO2) 纳米颗粒已经广泛应用于化妆品、 防晒霜、 涂料和牙膏等。 这些纳米颗粒性质非常稳定, 能在废水和生物固体中转移和分散。 现有研究表明, TiO2 纳米颗粒对动物正常生理活动具有毒性等负面作用。 但是, 它们对植物是否具有毒性特别是是否会产生植物基因毒性至今尚不清楚。 因此, 本文使用随机扩增多态性 DNA 技术研究 TiO2 纳米颗粒是否对西葫芦具有基因毒性, 为 TiO2 纳米颗粒排放进入环境后的潜在植物毒性风险评价提供依据。创新要点首次发现了 TiO2 纳米颗粒对西葫芦具有基因毒性。重要结论采用随机扩增多态性 DNA 技术, 发现 TiO2 纳米颗粒污染处理的西葫芦样品与未处理样品的基因组 DNA 图谱相比, 不仅在谱带强度有明显差异, 而且存在谱带消失和新谱带产生现象, 表明 TiO2 纳米颗粒对西葫芦具有基因毒性。
DOI: 10.1021/es903891g
发表时间: 2010-10-01
影响因子: 11.4
作者:
Lopez-Moreno, Martha L.;de la Rosa, Guadalupe;Hernandez-Viezcas, Jose A.;Castillo-Michel, Hiram;Botez, Cristian E.;Peralta-Videa, Jose R.;Gardea-Torresdey, Jorge L.
通讯作者: Gardea-Torresdey, Jorge L.
DOI: 10.1016/s0269-7491(02)00105-7
发表时间: 2002-01-01
影响因子: 8.9
作者:
Peralta-Videa, JR;Gardea-Torresdey, JL;Carrillo, G
通讯作者: Carrillo, G