Evidence of the differential biotransformation and genotoxicity of ZnO and CeO2 nanoparticles on soybean (Glycine max) plants.
Evidence of the differential biotransformation and genotoxicity of ZnO and CeO2 nanoparticles on soybean (Glycine max) plants.
复制标题
DOI：
10.1021/es903891g
复制
发表时间：
2010-10-01
影响因子：
11.4
通讯作者：
Gardea-Torresdey, Jorge L.
中科院分区：
文献类型：
--
作者：
Lopez-Moreno, Martha L.;de la Rosa, Guadalupe;Hernandez-Viezcas, Jose A.;Castillo-Michel, Hiram;Botez, Cristian E.;Peralta-Videa, Jose R.;Gardea-Torresdey, Jorge L.
科学界和公众对纳米材料对生物体影响的关注和兴趣都在增长。报告已经描述了纳米颗粒（NP）对微生物和大型生物（包括一些植物物种）的毒性。然而，据作者所知，还没有关于可食用陆生植物对纳米颗粒进行生物转化的报道。这里，首次显示，是有关植物幼苗中ZnO和CeO 2纳米颗粒生物转化的证据。虽然NPs不影响大豆发芽，但它们对植物生长和元素吸收产生了差异性影响。通过使用同步加速器X-射线吸收光谱，我们得到了明确的证据CeO 2纳米颗粒的存在下，在根，而ZnO纳米颗粒不存在。应用随机扩增多态性DNA技术检测纳米粒引起的DNA损伤和突变。从大豆植物暴露于CeO 2 NPs获得的结果显示，在2000 mg L-1处理下出现4条新条带，在4000 mg L-1处理下出现3条新条带。在这项研究中，我们证明了大豆植物暴露于CeO 2纳米颗粒的遗传毒性作用。
Concern and interest related to the effects of nanomaterials on living organisms are growing in both the scientific and public communities. Reports have described the toxicity of nanoparticles (NPs) on micro and macro organisms, including some plant species. Nevertheless, to the authors’ knowledge there are no reports on the biotransformation of NPs by edible terrestrial plants. Here, shown for the first time, is evidence pertaining to the biotransformation of ZnO and CeO2 NPs in plant seedlings. Although the NPs did not affect soybean germination, they produced a differential effect on plant growth and element uptake. By using synchrotron X-ray absorption spectroscopy we obtained clear evidence of the presence of CeO2 NPs in roots, whereas ZnO NPs were not present. Random amplified polymorphic DNA assay was applied to detect DNA damage and mutations caused by NPs. Results obtained from the exposure of soybean plants to CeO2 NPs show the appearance of four new bands at 2000 mg L−1 and three new bands at 4000 mg L−1 treatment. In this study we demonstrated genotoxic effects from the exposure of soybean plants to CeO2 NPs.
登录
查看更多内容
影响因子：
5.7
作者：
Bernal, M.;Cases, R.;Yruela, I.
通讯作者：
Yruela, I.
影响因子：
8.6
作者：
Shahin, AM;Grandjean, F;Schuman, TP
通讯作者：
Schuman, TP
影响因子：
8.9
作者：
Murakami, M.;Ae, N.;Ishikawa, S.
通讯作者：
Ishikawa, S.
影响因子：
4.5
作者：
Park, Eun-Jung;Choi, Jinhee;Park, Kwangsik
通讯作者：
Park, Kwangsik
影响因子：
5.8
作者：
Kasthuri, J.;Veerapandian, S.;Rajendiran, N.
通讯作者：
Rajendiran, N.