In vivo methods for imaging blood-brain barrier function and dysfunction.
In vivo methods for imaging blood-brain barrier function and dysfunction.
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DOI：
10.1007/s00259-022-05997-1
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发表时间：
2023-03
影响因子：
9.1
通讯作者：
Dickie, Ben Robert
中科院分区：
文献类型：
--
作者：
Harris, William James;Asselin, Marie-Claude;Hinz, Rainer;Parkes, Laura Michelle;Allan, Stuart;Schiessl, Ingo;Boutin, Herve;Dickie, Ben Robert
血脑屏障（BBB）是中枢神经系统和体循环之间的界面。它严格调节什么进入和从脑实质中移除，并且是维持脑内稳态的基础。越来越多地，BBB被认为在许多神经系统疾病中具有重要作用，从急性疾病（创伤性脑损伤、中风、癫痫发作）到慢性神经变性（阿尔茨海默病、血管性痴呆、小血管疾病）。已经开发了许多方法来在体外、体内和离体研究BBB。复杂的多细胞结构和疾病的影响难以在体外准确地重建，并且BBB的功能方面不能容易地离体研究。因此，研究完整BBB的体内方法的价值不能被夸大。这篇综述讨论了血脑屏障的结构和功能，以及这些是如何在疾病中受到影响。然后，它深入讨论了几个建立和新的方法在体内的BBB成像，重点是MRI，核成像，和高分辨率活体荧光显微镜。
The blood–brain barrier (BBB) is the interface between the central nervous system and systemic circulation. It tightly regulates what enters and is removed from the brain parenchyma and is fundamental in maintaining brain homeostasis. Increasingly, the BBB is recognised as having a significant role in numerous neurological disorders, ranging from acute disorders (traumatic brain injury, stroke, seizures) to chronic neurodegeneration (Alzheimer’s disease, vascular dementia, small vessel disease). Numerous approaches have been developed to study the BBB in vitro, in vivo, and ex vivo. The complex multicellular structure and effects of disease are difficult to recreate accurately in vitro, and functional aspects of the BBB cannot be easily studied ex vivo. As such, the value of in vivo methods to study the intact BBB cannot be overstated. This review discusses the structure and function of the BBB and how these are affected in diseases. It then discusses in depth several established and novel methods for imaging the BBB in vivo, with a focus on MRI, nuclear imaging, and high-resolution intravital fluorescence microscopy.
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