Cluster K mycobacteriophages: insights into the evolutionary origins of mycobacteriophage TM4.
Cluster K mycobacteriophages: insights into the evolutionary origins of mycobacteriophage TM4.
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DOI：
10.1371/journal.pone.0026750
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Hatfull GF
中科院分区：
文献类型：
--
作者：
Pope WH;Ferreira CM;Jacobs-Sera D;Benjamin RC;Davis AJ;DeJong RJ;Elgin SC;Guilfoile FR;Forsyth MH;Harris AD;Harvey SE;Hughes LE;Hynes PM;Jackson AS;Jalal MD;MacMurray EA;Manley CM;McDonough MJ;Mosier JL;Osterbann LJ;Rabinowitz HS;Rhyan CN;Russell DA;Saha MS;Shaffer CD;Simon SE;Sims EF;Tovar IG;Weisser EG;Wertz JT;Weston-Hafer KA;Williamson KE;Zhang B;Cresawn SG;Jain P;Piuri M;Jacobs WR Jr;Hendrix RW;Hatfull GF
五个新分离的分枝杆菌噬菌体-当归、CrimD、Adephagia、Anaya和Pixie-具有与分枝杆菌噬菌体TM4相似的基因组结构，TM4是一种以前被广泛用于分枝杆菌遗传学的噬菌体。核苷酸序列的相似性需要将它们归入簇K，并将其细分为三个亚簇：K1、K2和K3。虽然这些噬菌体的总体基因组结构相似，但TM4似乎丢失了至少两个基因组片段，一个是包含整合装置的中心区，另一个是右端的片段。这表明TM4是一种温带亲本的新近衍生物，解决了关于其生物学上的一个长期难题，据报道，它是从一种溶原性禽分枝杆菌菌株中回收的，但它不能在任何分枝杆菌宿主中形成溶原菌。和TM4一样，所有的K簇噬菌体都可以感染快速和缓慢生长的分枝杆菌，而且除了TM4之外，所有的噬菌体都在耻垢分枝杆菌和结核分枝杆菌中形成了稳定的溶原体；免疫分析表明，所有这五个噬菌体都具有相同的免疫特异性。TM4感染这些溶原菌，表明它要么来自异种免疫温带亲本，要么获得了毒力表型。我们还鉴定了一种广泛使用的TM4的条件复制衍生物，并鉴定了与温度敏感表型相关的突变。所有的K簇噬菌体都包含一系列与部分基因的翻译起始点相关的保守的13个核苷酸重复序列；其中大约一半包含一个额外的序列特征，由由可变间隔区分隔的不完全保守的17个核苷酸反向重复组成。K1噬菌体整合到宿主tmRNA中，而簇K噬菌体是结核分枝杆菌及其近缘物种遗传学研究的潜在新工具。
Five newly isolated mycobacteriophages –Angelica, CrimD, Adephagia, Anaya, and Pixie – have similar genomic architectures to mycobacteriophage TM4, a previously characterized phage that is widely used in mycobacterial genetics. The nucleotide sequence similarities warrant grouping these into Cluster K, with subdivision into three subclusters: K1, K2, and K3. Although the overall genome architectures of these phages are similar, TM4 appears to have lost at least two segments of its genome, a central region containing the integration apparatus, and a segment at the right end. This suggests that TM4 is a recent derivative of a temperate parent, resolving a long-standing conundrum about its biology, in that it was reportedly recovered from a lysogenic strain of Mycobacterium avium, but it is not capable of forming lysogens in any mycobacterial host. Like TM4, all of the Cluster K phages infect both fast- and slow-growing mycobacteria, and all of them – with the exception of TM4 – form stable lysogens in both Mycobacterium smegmatis and Mycobacterium tuberculosis; immunity assays show that all five of these phages share the same immune specificity. TM4 infects these lysogens suggesting that it was either derived from a heteroimmune temperate parent or that it has acquired a virulent phenotype. We have also characterized a widely-used conditionally replicating derivative of TM4 and identified mutations conferring the temperature-sensitive phenotype. All of the Cluster K phages contain a series of well conserved 13 bp repeats associated with the translation initiation sites of a subset of the genes; approximately one half of these contain an additional sequence feature composed of imperfectly conserved 17 bp inverted repeats separated by a variable spacer. The K1 phages integrate into the host tmRNA and the Cluster K phages represent potential new tools for the genetics of M. tuberculosis and related species.
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影响因子：
9.4
作者：
Eltringham, IJ;Wilson, SM;Drobniewski, FA
通讯作者：
Drobniewski, FA
影响因子：
2.8
作者：
Freitas-Vieira, A;Anes, E;Moniz-Pereira, J
通讯作者：
Moniz-Pereira, J
影响因子：
5.9
作者：
Hatfull, Graham F.;Hendrix, Roger W.
通讯作者：
Hendrix, Roger W.
影响因子：
2.8
作者：
Bardarov, S;Bardarov, S;Jacobs, WR
通讯作者：
Jacobs, WR
影响因子：
4.5
作者：
Hatfull GF;Pedulla ML;Jacobs-Sera D;Cichon PM;Foley A;Ford ME;Gonda RM;Houtz JM;Hryckowian AJ;Kelchner VA;Namburi S;Pajcini KV;Popovich MG;Schleicher DT;Simanek BZ;Smith AL;Zdanowicz GM;Kumar V;Peebles CL;Jacobs WR Jr;Lawrence JG;Hendrix RW
通讯作者：
Hendrix RW