Knockdown of myostatin expression by RNAi enhances muscle growth in transgenic sheep.
Knockdown of myostatin expression by RNAi enhances muscle growth in transgenic sheep.
复制标题
RNAi 抑制肌肉生长抑制素表达可增强转基因羊的肌肉生长
DOI：
10.1371/journal.pone.0058521
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Chen C
中科院分区：
文献类型：
--
作者：
Hu S;Ni W;Sai W;Zi H;Qiao J;Wang P;Sheng J;Chen C
肌生长抑制素（Myostatin，MSTs）是骨骼肌发育和生长的负调节因子。因此，哺乳动物功能障碍提供了一种在畜牧生产中促进动物生长性能的策略。在这项研究中，我们研究了利用基于RNAi的技术来产生具有双肌表型的转基因绵羊的可能性。使用靶向绵羊乳腺癌的shRNA表达盒来产生稳定的表达shRNA的成纤维细胞克隆。利用体细胞核移植（SCNT）技术进一步获得转基因绵羊。5只羔羊发育至足月，获得3只活羔羊。通过PCR证实shRNA表达盒在三只活羔羊中整合。RNase保护试验表明，靶向MSTs的shRNA在3只转基因绵羊的肌肉组织中均有表达。与对照羊相比，转基因羊肌肉组织中MSTK的表达受到显著抑制。此外，转基因羊表现出比对照羊更快地增加体重的趋势。组织学分析表明，转基因羊M17的肌纤维直径大于对照羊。我们的研究结果证明了一种很有前途的方法，以促进肌肉生长在畜牧生产。
Myostatin (MSTN) has been shown to be a negative regulator of skeletal muscle development and growth. MSTN dysfunction therefore offers a strategy for promoting animal growth performance in livestock production. In this study, we investigated the possibility of using RNAi-based technology to generate transgenic sheep with a double-muscle phenotype. A shRNA expression cassette targeting sheep MSTN was used to generate stable shRNA-expressing fibroblast clones. Transgenic sheep were further produced by somatic cell nuclear transfer (SCNT) technology. Five lambs developed to term and three live lambs were obtained. Integration of shRNA expression cassette in three live lambs was confirmed by PCR. RNase protection assay showed that the shRNAs targeting MSTN were expressed in muscle tissues of three transgenic sheep. MSTN expression was significantly inhibited in muscle tissues of transgenic sheep when compared with control sheep. Moreover, transgenic sheep showed a tendency to faster increase in body weight than control sheep. Histological analysis showed that myofiber diameter of transgenic sheep M17 were bigger than that of control sheep. Our findings demonstrate a promising approach to promoting muscle growth in livestock production.
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影响因子：
4.5
作者：
Mosher DS;Quignon P;Bustamante CD;Sutter NB;Mellersh CS;Parker HG;Ostrander EA
通讯作者：
Ostrander EA
影响因子：
3.7
作者：
Kong Q;Wu M;Huan Y;Zhang L;Liu H;Bou G;Luo Y;Mu Y;Liu Z
通讯作者：
Liu Z
影响因子：
2.5
作者：
Tessanne, K.;Golding, M. C.;Long, C. R.;Peoples, M. D.;Hannon, G.;Westhusin, M. E.
通讯作者：
Westhusin, M. E.
影响因子：
3.8
作者：
Fletcher, C. J.;Roberts, C. T.;McMillen, I. C.
通讯作者：
McMillen, I. C.
影响因子：
4.1
作者：
Acosta, J;Carpio, Y;Estrada, MP
通讯作者：
Estrada, MP