Characterization of serologically nontypeable Actinobacillus actinomycetemcomitans isolates.

Characterization of serologically nontypeable Actinobacillus actinomycetemcomitans isolates.
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血清学不可分型放线杆菌伴放线菌分离株的表征。

DOI:
10.1128/jcm.36.7.2019-2022.1998
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发表时间:
1998
影响因子:
9.4
通讯作者:
Asikainen,S
Asikainen,S
中科院分区:
医学2区
文献类型:
--
作者:
Paju,S;Saarela,M;Alaluusua,S;Fives-Taylor,P;Asikainen,S

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我们以前的研究表明,放线共放线杆菌分离物的任意引物PCR(AP-PCR)基因型属于同一血清型(血清型a至e)。在本研究中,我们调查了非致病型A.放线菌共生分离物与可分型的分离物相匹配。分离株的特征还在于限制性分析的theapaHPCR扩增产物。材料包括75个非典型和18个非典型A。放线菌共分离物来自34个流行病学无关的受试者。可分型的分离株来自也携带不可分型分离株的受试者。八个AP-PCR基因型之间的分离株进行了区分,六种基因型匹配,在我们以前的研究中检测到的,而两种基因型是新的。个人而言,A。在参与多个分离株的23名受试者中,有22名受试者的放线菌伴分离株产生了相同的AP-PCR带型,无论它们是血清型还是非血清型。AP-PCR基因型3,对应于血清型c,是迄今为止最常见的非分型分离株(62%的受试者)。apaH酶切分析结果证实了34例受试者中31例的AP-PCR结果。结果表明,非典型A.放线菌共生分离物来源于可分型的分离物,特别是来源于血清型C分离物,并且存在其它血清型的可能性很小。
Our previous studies have shown thatActinobacillus actinomycetemcomitansisolates of a given arbitrarily primed PCR (AP-PCR) genotype belong to the same serotype (of serotypes a through e). In the present study we investigated whether the AP-PCR genotypes of nonserotypeableA. actinomycetemcomitansisolates match those of the serotypeable isolates. The isolates were additionally characterized by restriction analysis of theapaHPCR amplification products. The material included 75 nonserotypeable and 18 serotypeableA. actinomycetemcomitansisolates from 34 epidemiologically unrelated subjects. The serotypeable isolates were obtained from subjects who also harbored nonserotypeable isolates. Eight AP-PCR genotypes were distinguished among the isolates; six genotypes matched those detected in our previous studies, whereas two genotypes were new. Intraindividually, theA. actinomycetemcomitansisolates produced identical AP-PCR banding patterns, regardless of whether they were serotypeable or nonserotypeable, in 22 of 23 subjects participating with multiple isolates. AP-PCR genotype 3, corresponding to serotype c, was by far the most common among the nonserotypeable isolates (62% of subjects). Results obtained with theapaHrestriction analysis confirmed the results obtained with AP-PCR for 31 of the 34 subjects. The results suggest that nonserotypeableA. actinomycetemcomitansisolates originate from serotypeable isolates, especially from serotype c isolates, and the likelihood of the existence of additional serotypes is small.
间接免疫荧光作为诊断工具,用于诊断由伴放线杆菌和表皮葡萄球菌引起的人工心脏瓣膜心内膜炎。
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影响因子: 3.5
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由伴放线放线杆菌引起的心包炎。
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期刊:
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