Differentiation of the serotype b and species‐specific antigens of Actinobacillus actinomycetemcomitans recognized by monoclonal antibodies
Differentiation of the serotype b and species‐specific antigens of Actinobacillus actinomycetemcomitans recognized by monoclonal antibodies
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单克隆抗体识别的伴放线杆菌的 b 血清型和种特异性抗原的区分
DOI：
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
K. Leung
中科院分区：
文献类型：
--
作者：
W. McArthur;S. Stroup;S. McClellan;K. Leung
血清b型抗原已被报道与脂多糖有关。利用b血清型抗原或放线菌属的小鼠单克隆抗体，确定了这两个表位与脂多糖的关系。种特异性和血清型b特异性单克隆抗体结合全细胞、囊泡和传统分离的脂多糖和多糖物质，这些物质来源于放线菌培养上清。血清型b特异性单克隆抗体结合的多糖酸水解脂多糖。种特异性单克隆抗体结合多糖和脂质A部分后的脂多糖酸水解。多粘菌素b部分抑制了种特异性单克隆抗体与脂多糖的结合，对血清型b特异性单克隆抗体与脂多糖的结合无影响。用脱氧胆酸凝胶过滤层析法将全菌脂多糖和培养上清中分离的多糖分成含有b血清型抗原、b血清型抗原和种特异性抗原或种特异性抗原的部分。sds -聚丙烯酰胺凝胶电泳和Western blotting分析表明，血清b型抗原位于高分子量的多糖材料上。物种特异性抗原位于低分子量多糖的阶梯上，与聚丙烯酰胺凝胶电泳分离的脂多糖分子的印迹模式相同，并用银染色。含有血清型b抗原的多糖化学分析显示85%的核糖，11%的葡萄糖，无脂质。物种特异性抗原物质的化学成分显示为典型的脂多糖组成。使用种特异性或血清b型特异性单克隆抗体进行免疫电镜检查，证实了两种抗原的生化和免疫学特性，表明种特异性抗原表位位于放线菌胞膜表面，血清b型特异性抗原表位位于细胞表面延伸的无定形物质上。结果表明，血清b型抗原可与脂多糖分离，为放线菌荚膜物质。种特异性抗原比血清型抗原更保守，存在于所有的脂多糖分子种上。
The serotype b antigens have been reported to be associated with lipopolysaccharide. Using murine monoclonal antibodies specific for either a serotype b antigen or the Actinobacillus actinomycetemcomitans species, the relationship of the two epitopes to lipopolysaccharide was determined. Both the species-specific and serotype b-specific monoclonal antibodies bound to whole cells, vesicles and conventionally isolated lipopolysaccharide and polysaccharide material derived from A. actinomycetemcomitans culture supernatants. Serotype b-specific monoclonal antibodies bound to the polysaccharide of acid-hydrolyzed lipopolysaccharide. Species-specific monoclonal antibodies bound to both the polysaccharide and the lipid A fraction of lipopolysaccharide after acid hydrolysis. Polymyxin b partially inhibited the binding of the species-specific monoclonal antibodies to lipopolysaccharide and had no effect on the binding of the serotype b-specific monoclonal antibodies to lipopolysaccharide. Lipopolysaccharide from whole bacteria and polysaccharide material isolated from culture supernatants were separated by gel filtration chromatography in deoxycholate into fractions that contained serotype b antigen, both serotype b and species-specific antigens, or species-specific antigen. SDS-polyacrylamide gel electrophoresis and Western blotting analysis of the fractions revealed that the serotype b antigen was on a high-molecular-weight polysaccharide material. The species-specific antigen was on a ladder of lower-molecular-weight polysaccharides identical to the blot pattern of lipopolysaccharide molecules separated by polyacrylamide gel electrophoresis and stained with silver stain. Chemical analysis of the polysaccharide containing serotype b antigen revealed 85% ribose, 11% glucose, and no lipid. Chemical content of the species-specific antigenic material revealed a composition typical of lipopolysaccharide. Immunoelectron microscopy using the species- or serotype b-specific monoclonal antibodies confirmed the biochemical and immunological characterization of the two antigens, showing that the species-specific epitopes were on the surface of the A. actinomycetemcomitans cell membrane and the serotype b-specific epitopes on the amorphous material extending from the cell surface. The data indicated that the serotype b antigen, detected by the antibody, was separable from lipopolysaccharide and was an A. actinomycetemcomitans capsular material. The species-specific antigen, being more conserved than the serotype antigen, was on all the lipopolysaccharide molecular species.
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影响因子：
6.7
作者：
Tsai,CC;McArthur,WP;Baehni,PC;Evian,C;Genco,RJ;Taichman,NS
通讯作者：
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由伴放线放线杆菌诱导的免疫抑制。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Shenker,BJ;McArthur,WP;Tsai,CC
通讯作者：
Tsai,CC
使用单克隆抗体对硝酸纤维素纸印迹上的放线放线杆菌分离株进行检测和血清分型。
影响因子：
6.7
作者：
McArthur,WP;Stroup,S;Wilson,L
通讯作者：
Wilson,L
伴放线放线杆菌对淋巴细胞反应的抑制。
影响因子：
3.5
作者：
Shenker,BJ;Tsai,CC;Taichman,NS
通讯作者：
Taichman,NS