Self-associated indisulam in phospholipid-based nanomicelles: a potential nanomedicine for cancer.
Self-associated indisulam in phospholipid-based nanomicelles: a potential nanomedicine for cancer.
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DOI：
10.1016/j.nano.2008.09.001
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发表时间：
2009-06
影响因子：
5.4
通讯作者：
Oenyueksel, Hayat
中科院分区：
文献类型：
--
作者：
Cesur, Hacer;Rubinstein, Israel;Pai, Ashwini;Oenyueksel, Hayat
关键词：
This study aims to start the development of a nanomedicine, containing indisulam solubilized in sterically stabilized micelles (SSM) composed of DSPE-PEG2000 or sterically stabilized mixed micelles (SSMM) composed of DSPE-PEG2000 plus egg phosphatidylcholine (EPC). Micelles were prepared by coprecipitation and reconstitution of drug and lipids. Particle size distributions of micellar formulations were determined by quasi-elastic light scattering (QELS). Amounts of solubilized drug were determined by Reverse Phase-High Performance Liquid Chromatography (RP-HPLC). In vitro cytotoxicity of indisulam in nanocarrier was determined on MCF-7 cell line by NCI developed Sulforhodamine B assay. Optimal solubilized indisulam concentrations in 5mM total lipid were 10 µg/ml for SSMM, and 400 µg/ml for SSM. HPLC results demonstrated encapsulation capacity of both micelles was over 95%. In vitro studies showed indisulam in micellar system was more effective than free indisulam. Optimized formulation was successfully freeze-dried without any addition of lyo- and cryo-protectants. We conclude SSM is a promising nanocarrier for indisulam, and indisulam-SSM should be developed further as a novel targeted nanomedicine.
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影响因子：
3.8
作者：
Ashok, B;Arleth, L;Önyüksel, H
通讯作者：
Önyüksel, H
影响因子：
50.5
作者：
Punt, CJA;Fumoleau, P;Campone, M
通讯作者：
Campone, M
影响因子：
10.8
作者：
Zhang, JX;Hansen, CB;Boch, R
通讯作者：
Boch, R
影响因子：
8.4
作者：
Ozawa, Y;Sugi, NH;Yoshimatsu, K
通讯作者：
Yoshimatsu, K
影响因子：
3.4
作者：
Hammad, MA;Müller, BW
通讯作者：
Müller, BW