Genetic manipulation of gut microbes enables single-gene interrogation in a complex microbiome.
Genetic manipulation of gut microbes enables single-gene interrogation in a complex microbiome.
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DOI：
10.1016/j.cell.2021.12.035
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发表时间：
2022-02-03
期刊：
影响因子：
64.5
通讯作者：
Guo CJ
中科院分区：
文献类型：
--
作者：
Jin WB;Li TT;Huo D;Qu S;Li XV;Arifuzzaman M;Lima SF;Shi HQ;Wang A;Putzel GG;Longman RS;Artis D;Guo CJ
Hundreds of microbiota genes are associated with host biology/disease. Unraveling the causal contribution of a microbiota gene to host biology remains difficult because many are encoded by non-model gut commensals and not genetically targetable. A general approach to identify their gene transfer methodology and build their gene manipulation tools would enable mechanistic dissections of their impact on host physiology. We developed a pipeline that identifies the gene transfer methods for multiple non-model microbes spanning 5 phyla, and we demonstrated the utility of their genetic tools by modulating microbiome-derived short-chain fatty acids and bile acids in vitro and in the host. In a proof-of-principle study, by deleting a commensal gene for bile acid synthesis in a complex microbiome, we discover an intriguing role of this gene in regulating colon inflammation. This technology will enable genetically engineering the non-model gut microbiome and facilitate mechanistic dissection of microbiota-host interactions. A pipeline for the genetic manipulation of non-model gut microbes enables single- gene, functional interrogation of bacteria in a complex microbial system
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影响因子：
32.4
作者：
Fung TC;Bessman NJ;Hepworth MR;Kumar N;Shibata N;Kobuley D;Wang K;Ziegler CGK;Goc J;Shima T;Umesaki Y;Sartor RB;Sullivan KV;Lawley TD;Kunisawa J;Kiyono H;Sonnenberg GF
通讯作者：
Sonnenberg GF
影响因子：
3.2
作者：
Bencivenga-Barry, Natasha A.;Lim, Bentley;Goodman, Andrew L.
通讯作者：
Goodman, Andrew L.
深入了解erysipelotrichaceae在人类宿主中的作用。
影响因子：
5.7
作者：
Kaakoush NO
通讯作者：
Kaakoush NO
影响因子：
14.8
作者：
Hmelo LR;Borlee BR;Almblad H;Love ME;Randall TE;Tseng BS;Lin C;Irie Y;Storek KM;Yang JJ;Siehnel RJ;Howell PL;Singh PK;Tolker-Nielsen T;Parsek MR;Schweizer HP;Harrison JJ
通讯作者：
Harrison JJ
影响因子：
6.4
作者：
Garcia-Bayona, Leonor;Comstock, Laurie E.
通讯作者：
Comstock, Laurie E.