Transbilayer movement of phosphatidylserine in nonhuman erythrocytes: evidence that the aminophospholipid transporter is a ubiquitous membrane protein.
Transbilayer movement of phosphatidylserine in nonhuman erythrocytes: evidence that the aminophospholipid transporter is a ubiquitous membrane protein.
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非人红细胞中磷脂酰丝氨酸的跨双层运动：氨基磷脂转运蛋白是普遍存在的膜蛋白的证据。
DOI：
10.1021/bi00451a021
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Schroit,AJ
中科院分区：
文献类型：
--
作者：
Connor,J;Schroit,AJ
Department of Cell Biology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030 Received May 10, 1989; Revised Manuscript Received August 2, 1989 abstract: A 31-32-kDa integral membrane protein has been previously identified in erythrocytes as the protein most likely to be responsible for the transbilayer movement of phosphatidylserine (PS)[Connor & Schroit (1988) Biochemistry 27, 848-851]. Using similar techniques, we have identified analogous proteins of identicalmolecular weights in bovine, equine, ovine, porcine, canine, caprine, and rhesus red blood cells. Similar to human red blood cells, all of the mammalian cells were able to specifically transport an exogenously supplied fluorescent PS analogue from their outer-to-inner membrane leaflet. In addition, transport could be reversibly inhibited with the sulfhydryl-specificinhibitor pyridyldithioethylamine (PDA). PDA-sensitive PS transport was also observed in nucleated human and murine celllines. Analysis of isolated plasma membranes from 125I-PDA-labeled cells revealed marked labeling of a 32 000-Da component. Attempts to inhibit PS transport by treating the cells with proteases, lectins, or antibody suggested that the 32-kDa polypeptide is an integral membrane protein that does notcontain sites critical to its function at the cell surface. e maintenance of a particular distribution of phospholipids between membrane bilayer leaflets appears to be a common property of eukaryotic cells. Whereasall phospholipids exhibit some degree of membrane asymmetry in nucleated cells (Etemadi, 1980; Op den Kamp, 1979), phosphatidylserine (PS) 1 is the only phospholipid which appears to be localized exclusively in the inner leaflet of both red blood cells (Verkleij et al., 1973; Gordesky et al., 1975) and resting platelets (Chap et al., 1977; Bevers et al., 1983). In red blood cells, this highly asymmetric organization of PS may be required for ho-meostasis; it has been suggested that increased exposure of PS in the cells’ outer leaflet, which occurs, for example, in sickle cells (Chiu et al., 1979; Lubin et al., 1981), may contribute to intravascular occlusion (Franck et al., 1985) and their removal by phagocytic cells (Schwartz et al., 1985). Activation of platelets, on the other hand, results in a reorientation of PS from the inner to outer leaflet (Bevers et al., 1982, 1983) which serves an important physiologic function inhemostasis by its participation in the coagulation cascade (Bevers et al., 1982; Rosing et al., 1985).Although the mechanism (s) responsible formaintaining membrane lipid asymmetry is (are) not known, recent studies using labeled lipid analogues have demonstrated in red blood cells (Seigneuret & Devaux, 1984; Tilley et al., 1986; Connor & Schroit, 1987), platelets (Sune et al., 1987), and nucleated cells (Martin & Pagano, 1987; Zachowski et al., 1987) that exogenously added PS analogues preferentially translocate from their site of insertion in the outer leaflet to the inner leaflet, thereby adopting an asymmetric distribution. This intrabilayer movement is protein mediated, since lipid transport is ATP dependent and temperature dependent and requires that membrane proteins be maintained in a reduced state
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影响因子：
3.5
作者：
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影响因子：
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DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Agre,P;Saboori,AM;Asimos,A;Smith,BL
通讯作者：
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DOI：
10.1016/0005-2736(78)90004-4
发表时间：
1978-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
K. Ridgwell;Marcel Tanner;D. Anstee
通讯作者：
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